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Plant Physiol, June 2000, Vol. 123, pp. 645-654
Calcium-Calmodulin Suppresses the Filamentous Actin-Binding
Activity of a 135-Kilodalton Actin-Bundling Protein Isolated from Lily
Pollen Tubes
Etsuo
Yokota,*
Shoshi
Muto, and
Teruo
Shimmen
Department of Life Science, Faculty of Science, Himeji Institute of
Technology, Harima Science Park City, Hyogo 678-1297, Japan (E.Y.,
T.S.); and BioScience Center, Nagoya University, Chikusa-ku, Nagoya
464-8601, Japan (S.M.)
We have isolated a 135-kD actin-bundling protein (P-135-ABP) from
lily (Lilium longiflorum) pollen tubes and have shown
that this protein is responsible for bundling actin filaments in lily pollen tubes (E. Yokota, K. Takahara, T. Shimmen [1998] Plant Physiol
116: 1421-1429). However, only a few thin actin-filament bundles are
present in random orientation in the tip region of pollen tubes, where
high concentrations of Ca2+ have also been found. To
elucidate the molecular mechanism for the temporal and spatial
regulation of actin-filament organization in the tip region of pollen
tubes, we explored the possible presence of factors modulating the
filamentous actin (F-actin)-binding activity of P-135-ABP. The
F-actin-binding activity of P-135-ABP in vitro was appreciably reduced
by Ca2+ and calmodulin (CaM), although neither
Ca2+ alone nor CaM in the presence of low concentrations of
Ca2+ affects the activity of P-135-ABP. A micromolar order
of Ca2+ and CaM were needed to induce the inhibition of the
binding activity of P-135-ABP to F-actin. An antagonist for CaM, W-7,
cancelled this inhibition. W-5 also alleviated the inhibition effect of Ca2+-CaM, however, more weakly than W-7. These results
suggest the specific interaction of P-135-ABP with
Ca2+-CaM. In the presence of both Ca2+ and CaM,
P-135-ABP organized F-actin into thin bundles, instead of the thick
bundles observed in the absence of CaM. These results suggest that the
inhibition of the P-135-ABP activity by Ca2+-CaM is an
important regulatory mechanism for organizing actin filaments in the
tip region of lily pollen tubes.
*
Corresponding author; e-mail yokota{at}sci.himeji-tech.ac.jp;
fax 0791-58-0175.
© 2000 American Society of Plant Physiologists
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