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Plant Physiol, July 2000, Vol. 123, pp. 1109-1120

Auxin Biosynthesis in Maize Kernels1

Erich Glawischnig,* Adriana Tomas, Wolfgang Eisenreich, Peter Spiteller, Adelbert Bacher, and Alfons Gierl

Lehrstuhl für Genetik (E.G., A.G.) and Lehrstuhl für Organische Chemie und Biochemie (W.E., A.B.), Technische Universität München, Lichtenbergstrasse 4, 85747 Garching, Germany; Pioneer Hi-Bred International, 7250 NW 62nd Avenue, Johnston, Iowa 50131-0552 (A.T.); and Institut für Organische Chemie, Ludwig-Maximilian-Universität München, Butenandstrasse 13, 81377 München, Germany (P.S.)

Auxin biosynthesis was analyzed in a maize (Zea mays) kernel culture system in which the seeds develop under physiological conditions similar to the in vivo situation. This system was modified for precursor feeding experiments. Tryptophan (Trp) is efficiently incorporated into indole-3-acetic acid (IAA) with retention of the 3,3' bond. Conversion of Trp to IAA is not competed by indole. Labeling with the general precursors [U-13C6]glucose and [1,2-13C2]acetate followed by retrobiosynthetic analysis strongly suggest that Trp-dependent IAA synthesis is the predominant route for auxin biosynthesis in the maize kernel. The synthesis of IAA from indole glycerol phosphate and IAA formation via condensation of indole with an acetyl-coenzyme A or phosphoenolpyruvate derived metabolite can be excluded.

1 This research was supported by the Deutsche Forschungsgemeinschaft (grant nos. SPP 1067 and SFB 369) and by Fonds der Chemischen Industrie.

* Corresponding author; e-mail mais{at}c2h5oh.org.chemie.tu-muenchen.de; fax 49-89-28912892.

© 2000 American Society of Plant Physiologists


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