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Plant Physiol, July 2000, Vol. 123, pp. 1173-1184

A Family of at Least Seven beta -Galactosidase Genes Is Expressed during Tomato Fruit Development

David L. Smith and Kenneth C. Gross*

Horticultural Crops Quality Laboratory, Agricultural Research Service, United States Department of Agriculture, Building 002, 10300 Baltimore Avenue, Beltsville, Maryland 20705-2350

During our search for a cDNA encoding beta -galactosidase II, a beta -galactosidase/exogalactanase (EC 3.2.1.23) present during tomato (Lycopersicon esculentum Mill.) fruit ripening, a family of seven tomato beta -galactosidase (TBG) cDNAs was identified. The shared amino acid sequence identity among the seven TBG clones ranged from 33% to 79%. All contained the putative active site-containing consensus sequence pattern G-G-P-[LIVM]-x-Q-x-E-N-E-[FY] belonging to glycosyl hydrolase family 35. Six of the seven single-copy genes were mapped using restriction fragment length polymorphisms of recombinant inbred lines. RNA gel-blot analysis was used to evaluate TBG mRNA levels throughout fruit development, in different fruit tissues, and in various plant tissues. RNA gel-blot analysis was also used to reveal TBG mRNA levels in fruit of the rin, nor, and Nr tomato mutants. The TBG4-encoded protein, known to correspond to beta -galactosidase II, was expressed in yeast and exo-galactanase activity was confirmed via a quantified release of galactosyl residues from cell wall fractions containing beta (1right-arrow4)-D-galactan purified from tomato fruit.

* Corresponding author; e-mail grossk{at}ba.ars.usda.gov; fax 301-504-5107.

© 2000 American Society of Plant Physiologists


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