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Plant Physiol, July 2000, Vol. 123, pp. 853-868
Cell-Specific and Conditional Expression of Caffeoyl-Coenzyme
A-3-O-Methyltransferase in Poplar1
Cuiying
Chen,
Hugo
Meyermans,
Bart
Burggraeve,
Riet M.
De Rycke,
Kentaro
Inoue,
Vera
De Vleesschauwer,
Marijke
Steenackers,
Marc C.
Van Montagu,
Gilbert J.
Engler, and
Wout A.
Boerjan*
Vakgroep Moleculaire Genetica & Departement Plantengenetica, Vlaams
Interuniversitair Instituut voor Biotechnologie (C.C., H.M., B.B.,
R.M.D.R., V.D.V., M.C.V.M., W.A.B.) and Laboratoire Associé de
l'Institut National de la Recherche Agronomique (France) (G.J.E.),
Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium; The
Samuel Roberts Noble Foundation, P.O. Box 2180, 2510 Sam Noble Parkway,
Ardmore, Oklahoma 73402 (K.I.); and Instituut voor Bosbouw en
Wildbeheer, Gaverstraat 4, B-9500 Geraardsbergen, Belgium
(M.S.)
Caffeoyl coenzyme A-3-O-methyltransferase (CCoAOMT)
plays an important role in lignin biosynthesis and is encoded by two
genes in poplar (Populus trichocarpa). Here, we describe
the expression pattern conferred by the two CCoAOMT
promoters when fused to the gus-coding sequence in
transgenic poplar (Populus tremula × Populus alba). Both genes were expressed similarly in xylem and
differentially in phloem. In xylem, expression was preferentially
observed in vessels and contact rays, whereas expression was barely
detectable in storage rays and fibers, suggesting different routes to
monolignol biosynthesis in the different xylem types. Furthermore,
after wounding, fungal infection, and bending, the expression of both genes was induced concomitantly with de novo lignin deposition. Importantly, upon bending and leaning of the stem, the cell-specific expression pattern was lost, and both genes were expressed in all cell
types of the xylem. CCoAOMT promoter activity correlated well with the presence of the CCoAOMT protein, as shown by
immunolocalization. These expression data may explain, at least in
part, the heterogeneity in lignin composition that is observed between
cell types and upon different environmental conditions.
1
This work was supported by the Flemish
Government (grant no. IBW/3/1998) and the European Union (grant nos.
FAIR CT95-0424 and INCO-DC IC18-CT97-0203). G.J.E. is a Research
Engineer of the Institut National de la Recherche Agronomique (France).
*
Corresponding author; e-mail woboe{at}gengenp.rug.ac.be; fax
32-9-2645349.
© 2000 American Society of Plant Physiologists
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