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Plant Physiol, July 2000, Vol. 123, pp. 869-882
Analysis of Gene Promoters for Two Tomato Polygalacturonases
Expressed in Abscission Zones and the Stigma
Seung-Beom
Hong,
Roy
Sexton, and
Mark L.
Tucker*
Soybean and Alfalfa Research Laboratory, United States Department
of Agriculture-Agricultural Research Service, Building 006, Beltsville Agricultural Research Center-West, 10300 Baltimore
Avenue, Beltsville, Maryland 20705 (S.-B.H., M.L.T.); and Department of
Biological Science, Stirling University, Stirling FK9 4LA,
United Kingdom (R.S.)
The tomato (Lycopersicon esculentum cv Ailsa Craig)
polygalacturonase genes TAPG1
(LYCes;Pga1;2) and TAPG4
(LYCes;Pga1;5) are abundantly expressed in both
abscission zones and the pistils of mature flowers. To further
investigate the spatial and temporal expression patterns for these
genes, the TAPG gene promoters were ligated to
 -glucuronidase (GUS) reporter genes and transformed into tomato. GUS
expression with both constructs was similar and entirely consistent
with the expression patterns of the native gene transcripts. GUS
activity was observed in the weakening abscission zones of the leaf
petiole, flower and fruit pedicel, flower corolla, and fruit calyx. In
leaf petiole and flower pedicel zones this activity was enhanced by
ethylene and inhibited by indole-3-acetic acid. On induction of
abscission with ethylene, GUS accumulation was much earlier in
TAPG4:GUS than in TAPG1:GUS transformants. Moreover, TAPG4:GUS staining
appeared to predominate in the vascular bundles relative to surrounding
cortex cells whereas TAPG1:GUS was more evenly distributed across the
separation layer. Like the native genes, GUS was also expressed in the
stigma. Activity was not apparent in pistils until the flowers had
opened and was confined to the stigma and style immediately proximal to
it. A minimal promoter construct consisting of a 247-bp 5'-upstream element from TAPG1 was found to be sufficient to direct
GUS expression in both abscission zones and the stigma.
*
Corresponding author; e-mail mtucker{at}asrr.arsusda.gov; fax
301-504-5728.
© 2000 American Society of Plant Physiologists
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