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Plant Physiol, July 2000, Vol. 123, pp. 905-916
CHRK1, a Chitinase-Related Receptor-Like Kinase in
Tobacco1
Youn Sung
Kim,2
Jeong Hee
Lee,
Gyeong Mee
Yoon,3
Hye Sun
Cho,
Seong-Whan
Park,4
Mi Chung
Suh,
Doil
Choi,
Hyun Jung
Ha,
Jang Ryol
Liu, and
Hyun-Sook
Pai*
Plant Cell Biotechnology Laboratory, Korea Research Institute of
Bioscience and Biotechnology, P.O. Box 115, Yusong, Taejon 305-600,
Korea (Y.S.K., J.H.L., G.M.Y., H.S.C., S.-W.P., D.C., J.R.L., H.-S.P.);
Graduate School of Biotechnology, Korea University, Seoul 136-701,
Korea (M.C.S.); and Division of Life Sciences, Chungbuk National
University, Cheongju 361-711, Korea (H.J.H.)
A cDNA encoding a chitinase-related receptor-like kinase,
designated CHRK1, was isolated from tobacco
(Nicotiana tabacum). The C-terminal kinase domain (KD)
of CHRK1 contained all of the conserved amino acids of serine/threonine
protein kinases. The putative extracellular domain was closely related
to the class V chitinase of tobacco and to microbial chitinases.
CHRK1 mRNA accumulation was strongly stimulated by
infection with fungal pathogen and tobacco mosaic virus. Amino
acid-sequence analysis revealed that the chitinase-like domain of CHRK1
lacked the essential glutamic acid residue required for
chitinase activity. The recombinant chitinase-like domain did not show
any catalytic activity for either oligomeric or polymeric chitin
substrates. The recombinant KD of CHRK1 exhibited autophosphorylation,
but the mutant KD with a mutation in the essential ATP-binding site did
not, suggesting that CHRK1 encoded a functional kinase. CHRK1 was
detected in membrane fractions of tobacco BY2 cells. Furthermore,
CHRK1-GFP fusion protein was localized in plasma membranes when it was
expressed in animal cells. This is the first report of a new type of
receptor-like kinase containing a chitinase-like sequence in the
putative extracellular domain.
1
This work was supported by a grant from Korea
Ministry of Agriculture and Forestry.
2
Present address: Center for Ligand and Transcription,
Chonnam National University, Kwangju 500-757, Korea.
3
Present address: Department of Horticulture, Washington
State University, Pullman, WA 99164-6414.
4
Present address: Department of Fruit Breeding, National
Horticulture Research Institute, Imok-dong 474, Changan-ku, Suwon, Kyungki-do 440-310, Korea.
*
Corresponding author; e-mail hyunsook{at}mail.kribb.re.kr; fax
82-42-860-4608.
© 2000 American Society of Plant Physiologists
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