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Plant Physiol, July 2000, Vol. 123, pp. 929-938
Identification of Phloem Involved in Assimilate Loading in
Leaves by the Activity of the Galactinol Synthase
Promoter1
Edith
Haritatos,
Brian G.
Ayre, and
Robert
Turgeon*
Department of Plant Biology, Cornell University, Ithaca, New York
14853
The definition of "minor" veins in leaves is arbitrary and of
uncertain biological significance. Generally, the term refers to the
smallest vein classes in the leaf, believed to function in phloem
loading. We found that a galactinol synthase promoter, cloned from
melon (Cucumis melo), directs expression of the
gusA gene to the smallest veins of mature Arabidopsis
and cultivated tobacco (Nicotiana tabacum) leaves. This
expression pattern is consistent with the role of galactinol synthase
in sugar synthesis and phloem loading in cucurbits. The expression
pattern in tobacco is especially noteworthy since galactinol is not
synthesized in the leaves of this plant. Also, we unexpectedly found
that expression in tobacco is limited to two of three companion cells
in class-V veins, which are the most extensive in the leaf. Thus, the
"minor" vein system is defined and regulated at the genetic level,
and there is heterogeneity of response to this system by different companion cells of the same vein.
1
This work was supported by the National Research
Initiative Competitive Grants Program/U.S. Department of
Agriculture (grant no. 9801617 to R.T.).
*
Corresponding author; e-mail ert2{at}cornell.edu; fax
607-255-5407.
© 2000 American Society of Plant Physiologists
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