|
Plant Physiol, July 2000, Vol. 123, pp. 987-996
Direct Measurement of Aluminum Uptake and Distribution in Single
Cells of Chara corallina1
Gregory J.
Taylor,*
Julie L.
McDonald-Stephens,
Douglas B.
Hunter,
Paul M.
Bertsch,
David
Elmore,
Zdenko
Rengel, and
Robert J.
Reid
Department of Biological Sciences, University of Alberta, Edmonton,
Alberta, Canada T6G 2E9 (G.J.T., J.L.M.-S.); Advanced Analytical Center
for Environmental Studies, Savannah River Ecology Laboratory, The
University of Georgia, Aiken, South Carolina 29801 (D.B.H.,
P.M.B.); Purdue Rare Isotope Measurement Laboratory, Purdue University,
West Lafayette, Indiana 47907-1396 (D.E.); Soil Science and Plant
Nutrition, Faculty of Agriculture, University of Western Australia,
Perth, Western Australia 6907, Australia (Z.R.); and Department of
Botany, University of Adelaide, Adelaide, South Australia 5005, Australia (R.J.R.)
Quantitative information on the uptake and distribution of Al at
the cellular level is required to understand mechanisms of Al toxicity,
but direct measurement of uptake across the plasma membrane has
remained elusive. We measured rates of Al transport across membranes in
single cells of Chara corallina using the rare
26Al isotope, an emerging technology (accelerator mass
spectrometry), and a surgical technique for isolating subcellular
compartments. Accumulation of Al in the cell wall dominated total
uptake (71-318 µg m 2 min1), although
transport across the plasma membrane was detectable (71-540 ng
m2 min1) within 30 min of exposure.
Transport across the tonoplast was initially negligible, but
accelerated to rates approximating uptake across the plasma membrane.
The avacuolate protoplasm showed signs of saturation after 60 min, but
continued movement across the plasma membrane was supported by
sequestration in the vacuole. Saturation of all compartments was
observed after 12 to 24 h. Accumulation of Al in the cell wall
reflected variation in {Al3+} induced by changes in Al
supply or complexing ligands, but was unaffected by pH. In contrast,
transport across the plasma membrane peaked at pH 4.3 and increased
when {Al3+} was reduced by complexing ligands. Cold
temperature (4°C) reduced accumulation in the cell wall and
protoplasm, whereas 2,4-dinitrophenol and
m-chlorocarbonylcyanidephenyl hydrazone increased membrane transport by 12- to 13-fold. Our data suggest that the cell wall is the
major site of Al accumulation. Nonetheless, membrane transport occurs
within minutes of exposure and is supported by subsequent sequestration
in the vacuole. The rapid delivery of Al to the protoplasm suggests
that intracellular lesions may be possible.
1
This research was supported by the Natural
Sciences and Engineering Research Council of Canada Collaborative
Project Grants Program, by the U.S. Department of Energy (grant no.
DE-FC09-96SR18546 to the University of Georgia Research Foundation),
by Southern California Edison, and by the University of Alberta Central
Research Fund.
*
Corresponding author; e-mail gregory.taylor{at}ualberta.ca; fax
780-492-9234.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
Y. Y. Li, J. L. Yang, Y. J. Zhang, and S. J. Zheng
Disorganized distribution of homogalacturonan epitopes in cell walls as one possible mechanism for aluminium-induced root growth inhibition in maize
Ann. Bot.,
August 1, 2009;
104(2):
235 - 241.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Babourina and Z. Rengel
Uptake of aluminium into Arabidopsis root cells measured by fluorescent lifetime imaging
Ann. Bot.,
July 1, 2009;
104(1):
189 - 195.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Illes, M. Schlicht, J. Pavlovkin, I. Lichtscheidl, F. Baluska, and M. Ovecka
Aluminium toxicity in plants: internalization of aluminium into cells of the transition zone in Arabidopsis root apices related to changes in plasma membrane potential, endosomal behaviour, and nitric oxide production
J. Exp. Bot.,
December 1, 2006;
57(15):
4201 - 4213.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Eticha, A. Stass, and W. J. Horst
Localization of aluminium in the maize root apex: can morin detect cell wall-bound aluminium?
J. Exp. Bot.,
May 1, 2005;
56(415):
1351 - 1357.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Doncheva, M. Amenos, C. Poschenrieder, and J. Barcelo
Root cell patterning: a primary target for aluminium toxicity in maize
J. Exp. Bot.,
April 1, 2005;
56(414):
1213 - 1220.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Facanha and A. L. Okorokova-Facanha
Inhibition of Phosphate Uptake in Corn Roots by Aluminum-Fluoride Complexes
Plant Physiology,
August 1, 2002;
129(4):
1763 - 1772.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yamamoto, Y. Kobayashi, S. R. Devi, S. Rikiishi, and H. Matsumoto
Aluminum Toxicity Is Associated with Mitochondrial Dysfunction and the Production of Reactive Oxygen Species in Plant Cells
Plant Physiology,
January 1, 2002;
128(1):
63 - 72.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Ahn, M. Sivaguru, H. Osawa, G. C. Chung, and H. Matsumoto
Aluminum Inhibits the H+-ATPase Activity by Permanently Altering the Plasma Membrane Surface Potentials in Squash Roots
Plant Physiology,
August 1, 2001;
126(4):
1381 - 1390.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Liu and S. Luan
Internal Aluminum Block of Plant Inward K+ Channels
PLANT CELL,
June 1, 2001;
13(6):
1453 - 1466.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Wenzl, G. M. Patiño, A. L. Chaves, J. E. Mayer, and I. M. Rao
The High Level of Aluminum Resistance in Signalgrass Is Not Associated with Known Mechanisms of External Aluminum Detoxification in Root Apices
Plant Physiology,
March 1, 2001;
125(3):
1473 - 1484.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Yamamoto, Y. Kobayashi, and H. Matsumoto
Lipid Peroxidation Is an Early Symptom Triggered by Aluminum, But Not the Primary Cause of Elongation Inhibition in Pea Roots
Plant Physiology,
January 1, 2001;
125(1):
199 - 208.
[Abstract]
[Full Text]
|
|
|
|
|
