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Plant Physiol, August 2000, Vol. 123, pp. 1257-1268

Farnesol-Induced Cell Death and Stimulation of 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase Activity in Tobacco cv Bright Yellow-2 Cells1,2

Andréa Hemmerlin and Thomas J. Bach*

Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire des Plantes, Département Isoprénoïdes, Institut de Botanique, Université Louis Pasteur, 28 rue Goethe, F-67083 Strasbourg, France

Growth inhibition of tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells by mevinolin, a specific inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) could be partially overcome by the addition of farnesol. However, farnesol alone inhibited cell division and growth as measured by determination of fresh weight increase. When 7-d-old tobacco cv Bright Yellow-2 cells were diluted 40-fold into fresh culture, the cells exhibited a dose-dependent sensitivity to farnesol, with 25 µM sufficient to cause 100% cell death, as measured by different staining techniques, cytometry, and monitoring of fragmentation of genomic DNA. Cells were less sensitive to the effects of farnesol when diluted only 4-fold. Farnesol was absorbed by the cells, as examined by [1-3H]farnesol uptake, with a greater relative enrichment by the more diluted cells. Both mevinolin and farnesol treatments stimulated apparent HMGR activity. The stimulation by farnesol was also reflected in corresponding changes in the steady-state levels of HMGR mRNA and enzyme protein with respect to HMGR gene expression and enzyme protein accumulation.

1 A part of this study was made possible by a Ph.D. fellowship of the Ministère de l'Education Nationale, de l'Enseignement Supérieur et de la Recherche to A.H.

2 Parts of the results of this study were presented at the Annual Meeting of the American Society of Plant Physiologists, Vancouver, August 2-6, 1997.

* Corresponding author; e-mail Thomas.Bach{at}bota-ulp.ustrasbg.fr; fax 33-3-88-35-84-84.

© 2000 American Society of Plant Physiologists


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