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Plant Physiol, August 2000, Vol. 123, pp. 1257-1268
Farnesol-Induced Cell Death and Stimulation of
3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase Activity in Tobacco
cv Bright Yellow-2 Cells1,2
Andréa
Hemmerlin and
Thomas J.
Bach*
Centre National de la Recherche Scientifique, Institut de Biologie
Moléculaire des Plantes, Département
Isoprénoïdes, Institut de Botanique, Université
Louis Pasteur, 28 rue Goethe, F-67083 Strasbourg, France
Growth inhibition of tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells by mevinolin, a specific inhibitor of
3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) could be
partially overcome by the addition of farnesol. However, farnesol alone
inhibited cell division and growth as measured by determination of
fresh weight increase. When 7-d-old tobacco cv Bright Yellow-2 cells
were diluted 40-fold into fresh culture, the cells exhibited a
dose-dependent sensitivity to farnesol, with 25 µM
sufficient to cause 100% cell death, as measured by different staining
techniques, cytometry, and monitoring of fragmentation of genomic DNA.
Cells were less sensitive to the effects of farnesol when diluted only
4-fold. Farnesol was absorbed by the cells, as examined by
[1-3H]farnesol uptake, with a greater relative enrichment
by the more diluted cells. Both mevinolin and farnesol treatments
stimulated apparent HMGR activity. The stimulation by farnesol was also
reflected in corresponding changes in the steady-state levels of HMGR
mRNA and enzyme protein with respect to HMGR gene expression and enzyme protein accumulation.
1
A part of this study was made possible by a
Ph.D. fellowship of the Ministère de l'Education Nationale, de
l'Enseignement Supérieur et de la Recherche to A.H.
2
Parts of the results of this study were
presented at the Annual Meeting of the American Society of Plant
Physiologists, Vancouver, August 2-6, 1997.
*
Corresponding author; e-mail Thomas.Bach{at}bota-ulp.ustrasbg.fr;
fax 33-3-88-35-84-84.
© 2000 American Society of Plant Physiologists
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