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Plant Physiol, August 2000, Vol. 123, pp. 1399-1414
Limited Correlation between Expansin Gene Expression and
Elongation Growth Rate1
Doina
Caderas,
Matthias
Muster,
Hannes
Vogler,
Therese
Mandel,
Jocelyn K.C.
Rose,
Simon
McQueen-Mason, and
Cris
Kuhlemeier*
Institute of Plant Physiology, University of Berne, Altenbergrain
21, CH-3013 Berne, Switzerland (D.C., M.M., H.V., T.M., C.K.);
Complex Carbohydrate Research Center, University of Georgia, Athens,
Georgia 30602-4712 (J.K.C.R.); and The Plant Laboratory, Department of
Biology, University of York, Heslington, York YO1 5YW, United Kingdom
(S.M.-M.)
The aim of this work was to study the role of the cell wall protein
expansin in elongation growth. Expansins increase cell wall
extensibility in vitro and are thought to be involved in cell
elongation. Here, we studied the regulation of two tomato (Lycopersicon esculentum cv Moneymaker) expansin genes,
LeExp2 and LeExp18, in rapidly expanding
tissues. LeExp2 was strongly expressed in the elongation
zone of hypocotyls and in the faster growing stem part during
gravitropic stimulation. LeExp18 expression did not
correlate with elongation growth. Exogenous application of hormones
showed a substantial auxin-stimulation of LeExp2 mRNA in
etiolated hypocotyls and a weaker auxin-stimulation of
LeExp18 mRNA in stem tissue. Analysis of transcript
accumulation revealed higher levels of LeExp2 and
LeExp18 in light-treated, slow-growing tissue than in
dark-treated, rapidly elongating tissue. Expansin protein levels and
cell wall extension activities were similar in light- and dark-grown
hypocotyl extracts. The results show a strong correlation between
expansin gene expression and growth rate, but this correlation is not
absolute. We conclude that elongation growth is likely to be controlled
by expansin acting in concert with other factors that may limit growth
under some physiological conditions.
1
This work was supported by the Swiss National
Science Foundation (grant no. 3100-045511.95).
*
Corresponding author; e-mail cris.kuhlemeier{at}pfp.unibe.ch; fax
41-31-332-2059.
© 2000 American Society of Plant Physiologists
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