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Plant Physiol, August 2000, Vol. 123, pp. 1507-1516
Elicitation of Suspension-Cultured Tomato Cells Triggers the
Formation of Phosphatidic Acid and Diacylglycerol
Pyrophosphate1
Arnold H.
van der Luit,2
Titus
Piatti,3
Aveline
van Doorn,
Alan
Musgrave,
Georg
Felix,
Thomas
Boller, and
Teun
Munnik*
Swammerdam Institute for Life Sciences, Department of Plant
Physiology, University of Amsterdam, Kruislaan 318, NL-1098 SM
Amsterdam, Netherlands (A.H.v.d.L., A.v.D., A.M., T.M.); and Friedrich
Miescher-Institute, P.O.B. 2543, CH-4002 Basel, Switzerland (T.P.,
G.F., T.B.)
Phosphatidic acid (PA) and its phosphorylated derivative
diacylglycerol pyrophosphate (DGPP) are lipid molecules that have been
implicated in plant cell signaling. In this study we report the rapid
but transient accumulation of PA and DGPP in suspension-cultured tomato
(Lycopersicon esculentum) cells treated with the general elicitors,
N,N',N",N -tetraacetylchitotetraose,
xylanase, and the flagellin-derived peptide flg22. To determine whether
PA originated from the activation of phospholipase D or from the
phosphorylation of diacylglycerol (DAG) by DAG kinase, a strategy
involving differential radiolabeling with
[32P]orthophosphate was used. DAG kinase was found to be
the dominant producer of PA that was subsequently metabolized to DGPP.
A minor but significant role for phospholipase D could only be detected when xylanase was used as elicitor. Since PA formation was correlated with the high turnover of polyphosphoinositides, we hypothesize that
elicitor treatment activates phospholipase C to produce DAG, which in
turn acts as substrate for DAG kinase. The potential roles of PA and
DGPP in plant defense signaling are discussed.
1
This work was financed by the Netherlands
Organization of Scientific Research (grant no. 805-33-232). T.M.
is funded by the Royal Netherlands Academy of Arts and Sciences and the
Netherlands Organization for Scientific Research (grant no. NWO-PULS
805-48-005).
2
Present Address: Division of Cellular Biochemistry, The
Netherlands Cancer Institute, Plesmanlaan 121, NL-1066 CX, Amsterdam, The Netherlands.
3
This manuscript is dedicated to the memory of Titus
Piatti, who died prior to the publication of this paper.
*
Corresponding author; e-mail munnik{at}bio.uva.nl; fax
31-20-5257934.
© 2000 American Society of Plant Physiologists
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