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Plant Physiol, August 2000, Vol. 123, pp. 1611-1622
Induction of a C4-Like Mechanism of CO2
Fixation in Egeria densa, a Submersed Aquatic
Species1
Paula
Casati,2
María V.
Lara,2 and
Carlos S.
Andreo*
Centro de Estudios Fotosintéticos y Bioquímicos,
Universidad Nacional de Rosario, Consejo Nacional de Investigaciones
Científicas y Técnicas, Suipacha 531, 2000 Rosario,
Argentina
The expression of phosphoenolpyruvate carboxylase
(PEPC) and NADP-malic enzyme (NADP-ME) in Egeria densa
leaves was studied under low temperature and light (LTL) following
incubation under high temperature and light (HTL), conditions
previously shown to induce high and low CO2 compensation
points, respectively. Transfer from LTL to HTL conditions induced
increases in the activities and amounts of both enzymes. One NADP-ME
isoform was observed in induced and uninduced samples. Two isoforms of
PEPC were expressed, with the lower Mr
isoform being induced by HTL. NADP-ME showed properties similar to
those of the isoform in C3 species. The inducible PEPC
isoform has a low Km for both substrates.
PEPC kinetic and regulatory properties (Vmax
and Km for
phosphoenolpyruvate, and I50
for L-malate) are different in samples taken in the dark from those in the light, indicating that some modification of PEPC may
be occurring during the day. Finally, abscisic acid induced the
expression of PEPC and NADP-ME in a manner similar to temperature induction, except that the activities of both PEPC isoforms were increased. A different signaling system may exist in this species in
response to high temperature or abscisic acid, both of which induce
changes in photosynthetic metabolism.
1
This work was funded by grants from the Consejo
Nacional de Investigaciones Científicas y Técnicas
(CONICET) and from the Agencia Nacional de Promoción
Científica y Tecnológica (BID 802/0C-AR PICT no.
1-03397). P.C. and M.V.L. are fellows from CONICET and C.S.A. is a
Researcher Career Member of the same institution.
2
These authors contributed equally to this work.
*
Corresponding author; e-mail candreo{at}agatha.unr.edu.ar; fax
54-341-4370044.
© 2000 American Society of Plant Physiologists
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