|
Plant Physiol, September 2000, Vol. 124, pp. 105-114
Trehalose Induces the ADP-Glucose Pyrophosphorylase Gene,
ApL3, and Starch Synthesis in
Arabidopsis1
Astrid
Wingler,*
Thorsten
Fritzius,
Andres
Wiemken,
Thomas
Boller, and
Roger A.
Aeschbacher
Botanisches Institut, Universität Basel, Hebelstrasse 1, CH-4056 Basel, Switzerland
In Arabidopsis, genes encoding functional enzymes for the synthesis
and degradation of trehalose have been detected recently. In this study
we analyzed how trehalose affects the metabolism and development of
Arabidopsis seedlings. Exogenously applied trehalose (25 mM) strongly reduced the elongation of the roots and,
concomitantly, induced a strong accumulation of starch in the shoots,
whereas the contents of soluble sugars were not increased. When
Arabidopsis seedlings were grown on trehalose plus sucrose (Suc), root
elongation was restored, but starch still accumulated to a much larger
extent than during growth on Suc alone. The accumulation of starch in
the shoots of trehalose-treated seedlings was accompanied by an
increased activity of ADP-glucose pyrophosphorylase and an induction of
the expression of the ADP-glucose pyrophosphorylase gene,
ApL3. Even in the presence of 50 mM Suc,
which itself also slightly induced ApL3, trehalose (5 mM) led to a further increase in ApL3
expression. These results suggest that trehalose interferes with carbon
allocation to the sink tissues by inducing starch synthesis in the
source tissues. Furthermore, trehalose induced the expression of the
 -amylase gene, AT--Amy, in
combination with Suc but not when trehalose was supplied alone,
indicating that trehalose can modulate sugar-mediated gene expression.
1
This work was supported by the Swiss National
Science Foundation (grant no. 3100-042535.94 to A.W. and grant no.
3100-040837.94 to T.B.).
*
Corresponding author; e-mail astrid.wingler{at}unibas.ch; fax
41-61-2672330.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
G. E. Welbaum, Z.-X. Shen, J. I. Watkinson, C.-L. Wang, and J. Nowak
Priming Soilless Growing Medium with Disaccharides Stimulated Microbial Biofilm Formation, and Increased Particle Aggregation and Moisture Retention during Muskmelon Transplant Production
J. Amer. Soc. Hort. Sci.,
May 1, 2009;
134(3):
387 - 395.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-y. Wu, A. Trieu, P. Radhakrishnan, S. F. Kwok, S. Harris, K. Zhang, J. Wang, J. Wan, H. Zhai, S. Takatsuto, et al.
Brassinosteroids Regulate Grain Filling in Rice
PLANT CELL,
August 1, 2008;
20(8):
2130 - 2145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Suzuki, S. Bajad, J. Shuman, V. Shulaev, and R. Mittler
The Transcriptional Co-activator MBF1c Is a Key Regulator of Thermotolerance in Arabidopsis thaliana
J. Biol. Chem.,
April 4, 2008;
283(14):
9269 - 9275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. N. Sokolov, J. R. Dominguez-Solis, A.-L. Allary, B. B. Buchanan, and S. Luan
A redox-regulated chloroplast protein phosphatase binds to starch diurnally and functions in its accumulation
PNAS,
June 20, 2006;
103(25):
9732 - 9737.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Crevillen, T. Ventriglia, F. Pinto, A. Orea, A. Merida, and J. M. Romero
Differential Pattern of Expression and Sugar Regulation of Arabidopsis thaliana ADP-glucose Pyrophosphorylase-encoding Genes
J. Biol. Chem.,
March 4, 2005;
280(9):
8143 - 8149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Avonce, B. Leyman, J. O. Mascorro-Gallardo, P. Van Dijck, J. M. Thevelein, and G. Iturriaga
The Arabidopsis Trehalose-6-P Synthase AtTPS1 Gene Is a Regulator of Glucose, Abscisic Acid, and Stress Signaling
Plant Physiology,
November 1, 2004;
136(3):
3649 - 3659.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Schluepmann, A. van Dijken, M. Aghdasi, B. Wobbes, M. Paul, and S. Smeekens
Trehalose Mediated Growth Inhibition of Arabidopsis Seedlings Is Due to Trehalose-6-Phosphate Accumulation
Plant Physiology,
June 1, 2004;
135(2):
879 - 890.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I.-C. Jang, S.-J. Oh, J.-S. Seo, W.-B. Choi, S. I. Song, C. H. Kim, Y. S. Kim, H.-S. Seo, Y. D. Choi, B. H. Nahm, et al.
Expression of a Bifunctional Fusion of the Escherichia coli Genes for Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase in Transgenic Rice Plants Increases Trehalose Accumulation and Abiotic Stress Tolerance without Stunting Growth
Plant Physiology,
February 1, 2003;
131(2):
516 - 524.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Eastmond, Y. Li, and I. A. Graham
Is trehalose-6-phosphate a regulator of sugar metabolism in plants?
J. Exp. Bot.,
January 3, 2003;
54(382):
533 - 537.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Rolland, B. Moore, and J. Sheen
Sugar Sensing and Signaling in Plants
PLANT CELL,
May 1, 2002;
14(90001):
S185 - 205.
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Vogel, O. Fiehn, L. Jean-Richard-dit-Bressel, T. Boller, A. Wiemken, R. A. Aeschbacher, and A. Wingler
Trehalose metabolism in Arabidopsis: occurrence of trehalose and molecular cloning and characterization of trehalose-6-phosphate synthase homologues
J. Exp. Bot.,
September 1, 2001;
52(362):
1817 - 1826.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Fritzius, R. Aeschbacher, A. Wiemken, and A. Wingler
Induction of ApL3 Expression by Trehalose Complements the Starch-Deficient Arabidopsis Mutant adg2-1 Lacking ApL1, the Large Subunit of ADP-Glucose Pyrophosphorylase
Plant Physiology,
June 1, 2001;
126(2):
883 - 889.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Müller, R. A. Aeschbacher, A. Wingler, T. Boller, and A. Wiemken
Trehalose and Trehalase in Arabidopsis
Plant Physiology,
February 1, 2001;
125(2):
1086 - 1093.
[Abstract]
[Full Text]
|
|
|
|
|
