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Plant Physiol, September 2000, Vol. 124, pp. 125-134

Expression of Arabidopsis CAX2 in Tobacco. Altered Metal Accumulation and Increased Manganese Tolerance1

Kendal D. Hirschi,2* Victor D. Korenkov,3 Nathaniel L. Wilganowski, and George J. Wagner

Baylor College of Medicine, Plant Physiology Group, United States Department of Agriculture/Agricultural Research Service Children's Nutrition Research Center, Houston, Texas 77030 (K.D.H., N.L.W.); and Plant Physiology/Biochemistry/Molecular Biology Program, Department of Agronomy, University of Kentucky, Lexington, Kentucky 40546-0091 (V.D.K., G.J.W.)

Metal transport from the cytosol to the vacuole is thought to be an important component of ion tolerance and of a plant's potential for use in phytoremediation. The Arabidopsis antiporter CAX2 (calcium exchanger 2) may be a key mediator of this process. CAX2 expression in yeast suppressed both Ca2+ and Mn2+ growth defects. A peptide-specific antibody to the antiporter reacted with a 39-kD protein from plant vacuolar membranes. Tobacco (Nicotiana tabacum) plants expressing CAX2 accumulated more Ca2+, Cd2+, and Mn2+ and were more tolerant to elevated Mn2+ levels. Expression of CAX2 in tobacco increased Cd2+ and Mn2+ transport in isolated root tonoplast vesicles. These results suggest that CAX2 has a broad substrate range and modulation of this transporter may be an important component of future strategies to improve plant ion tolerance.

1 This work was supported in part by the National Institutes of Health (grant nos. CHRC 5 P30 and 1R01 GM 57427) and by the U.S. Department of Agriculture/Agricultural Research Service under cooperative agreement (grant no. 58-6250-6001).

2 K.D.H. would like to dedicate this work to the memory of Billy Hahn.

3 Present address: K.A. Timiriazeu Institute of Plant Physiology RAS, Moscow, Russia.

* Corresponding author; e-mail kendalh{at}bcm.tmc.edu; fax 713-798-7078.

© 2000 American Society of Plant Physiologists


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