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Plant Physiol, September 2000, Vol. 124, pp. 135-152
Redistribution of Golgi Stacks and Other Organelles
during Mitosis and Cytokinesis in Plant
Cells1,[w]
Andreas
Nebenführ,*
Jennifer A.
Frohlick, and
L.
Andrew
Staehelin
Department of Molecular, Cellular and Developmental Biology,
University of Colorado, Boulder, Colorado 80309
We have followed the redistribution of Golgi stacks during mitosis
and cytokinesis in living tobacco BY-2 suspension culture cells by
means of a green fluorescent protein-tagged soybean  -1,2 mannosidase, and correlated the findings to cytoskeletal rearrangements and to the redistribution of endoplasmic reticulum, mitochondria, and
plastids. In preparation for cell division, when the general streaming
of Golgi stacks stops, about one-third of the peripheral Golgi stacks
redistributes to the perinuclear cytoplasm, the phragmosome, thereby
reversing the ratio of interior to cortical Golgi from 2:3 to 3:2.
During metaphase, approximately 20% of all Golgi stacks aggregate in
the immediate vicinity of the mitotic spindle and a similar number
becomes concentrated in an equatorial region under the plasma membrane.
This latter localization, the "Golgi belt," accurately predicts the
future site of cell division, and thus forms a novel marker for this
region after the disassembly of the preprophase band. During telophase
and cytokinesis, many Golgi stacks redistribute around the phragmoplast
where the cell plate is formed. At the end of cytokinesis, the daughter
cells have very similar Golgi stack densities. The sites of
preferential Golgi stack localization are specific for this organelle
and largely exclude mitochondria and plastids, although some
mitochondria can approach the phragmoplast. This segregation of
organelles is first observed in metaphase and persists until completion
of cytokinesis. Maintenance of the distinct localizations does not depend on intact actin filaments or microtubules, although the mitotic
spindle appears to play a major role in organizing the organelle
distribution patterns. The redistribution of Golgi stacks during
mitosis and cytokinesis is consistent with the hypothesis that Golgi
stacks are repositioned to ensure equal partitioning between daughter
cells as well as rapid cell plate assembly.
1
This work was supported by the National
Institutes of Health (grant no. GM18639 to L.A.S.).
[w]
Indicates Web-only data.
*
Corresponding author; e-mail andreas.nebenfuehr{at}colorado.edu;
fax 303-492-7744.
© 2000 American Society of Plant Physiologists
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