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Plant Physiol, September 2000, Vol. 124, pp. 191-200
Radial Distribution Pattern of Pectin Methylesterases across
the Cambial Region of Hybrid Aspen at Activity and
Dormancy1
Fabienne
Micheli,*
Björn
Sundberg,
Renée
Goldberg, and
Luc
Richard
Laboratoire d'Enzymologie en Milieu Structuré,
Département de Biologie Supramoléculaire et Cellulaire,
Institut Jacques Monod, 2 Place Jussieu, F-75252 Paris cedex 05, France (F.M., R.G., L.R.); Unité de Physiologie Cellulaire et
Moléculaire des Plantes, Université Pierre et Marie Curie,
Unité Mixte de Recherche 7632 Centre National de la Recherche
Scientifique, 4 Place Jussieu, Case 154, F-75252 Paris cedex 05, France (F.M., R.G., L.R.); and Department of Forest Genetics and Plant
Physiology, Swedish University of Agricultural Sciences, 90183 Umeå, Sweden (B.S.)
Biochemical microanalysis combined with tangential cryosectioning
was used to visualize the distribution of pectin methylesterases (PMEs)
across the cambial region in active and dormant hybrid aspen
(Populus tremula L. × Populus
tremuloides Michx). These novel techniques allowed us to relate
activity and isoforms of PMEs to specific tissues and developmental
stages of the stem to get more information on the physiological
function of PMEs in cambial growth. Isoelectrofocusing analysis
revealed numerous isoforms that were differentially distributed
according to the tissue-type and to the cambial stage. A neutral
isoform was found to be distributed ubiquitously across the stem of
both active and dormant trees, which suggests that it is a housekeeping
isoform involved in the maintenance of the cell wall integrity
throughout the stem. In addition, two distinct isoforms having
different isoelectric points were found to be related to the
differentiation of cambial derivatives. A basic isoform appears to be a
physiological marker of the dormant stage involved in the cessation of
meristematic radial growth, whereas an acidic isoform is functionally
related to the immediate expansion of the cambial daughter cells that occurs bilaterally on each side of the cambium at the active stage.
1
This research was supported by a grant from the
commission of the European Communities, Agriculture and Fisheries'
Research and Technological Development programme (no. CT 98-3972)
entitled "Wood formation processes: the key to improvement of the raw
material."
*
Corresponding author; e-mail micheli{at}ijm.jussieu.fr; fax
33-1- 44-27-61-51.
© 2000 American Society of Plant Physiologists
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