Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (49)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cahoon, E. B.
Right arrow Articles by Kinney, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cahoon, E. B.
Right arrow Articles by Kinney, A. J.
Agricola
Right arrow Articles by Cahoon, E. B.
Right arrow Articles by Kinney, A. J.

Plant Physiol, September 2000, Vol. 124, pp. 243-252

Production of Fatty Acid Components of Meadowfoam Oil in Somatic Soybean Embryos

Edgar B. Cahoon, Elizabeth-France Marillia, Kevin L. Stecca, Sarah E. Hall, David C. Taylor, and Anthony J. Kinney*

DuPont Nutrition and Health, Experimental Station, Wilmington, Delaware 19880-0402 (E.B.C., K.L.S., S.E.H., A.J.K.); and National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon, Saskatchewan, Canada S7N 0W9 (E.-F.M., D.C.T.)

The seed oil of meadowfoam (Limnanthes alba) and other Limnanthes spp. is enriched in the unusual fatty acid Delta 5-eicosenoic acid (20:1Delta 5). This fatty acid has physical and chemical properties that make the seed oil of these plants useful for a number of industrial applications. An expressed sequence tag approach was used to identify cDNAs for enzymes involved in the biosynthesis of 20:1Delta 5). By random sequencing of a library prepared from developing Limnanthes douglasii seeds, a class of cDNAs was identified that encode a homolog of acyl-coenzyme A (CoA) desaturases found in animals, fungi, and cyanobacteria. Expression of a cDNA for the L. douglasii acyl-CoA desaturase homolog in somatic soybean (Glycine max) embryos behind a strong seed-specific promoter resulted in the accumulation of Delta 5-hexadecenoic acid to amounts of 2% to 3% (w/w) of the total fatty acids of single embryos. Delta 5-Octadecenoic acid and 20:1Delta 5 also composed <1% (w/w) each of the total fatty acids of these embryos. In addition, cDNAs were identified from the L. douglasii expressed sequence tags that encode a homolog of fatty acid elongase 1 (FAE1), a beta -ketoacyl-CoA synthase that catalyzes the initial step of very long-chain fatty acid synthesis. Expression of the L. douglassi FAE1 homolog in somatic soybean embryos was accompanied by the accumulation of C20 and C22 fatty acids, principally as eicosanoic acid, to amounts of 18% (w/w) of the total fatty acids of single embryos. To partially reconstruct the biosynthetic pathway of 20:1Delta 5 in transgenic plant tissues, cDNAs for the L. douglasii acyl-CoA desaturase and FAE1 were co-expressed in somatic soybean embryos. In the resulting transgenic embryos, 20:1Delta 5 and Delta 5-docosenoic acid composed up to 12% of the total fatty acids.

* Corresponding author; e-mail anthony.kinney{at}usa.dupont.com; fax 703-935-4482.

© 2000 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
O. Sayanova, R. Haslam, M. Venegas Caleron, and J. A. Napier
Cloning and Characterization of Unusual Fatty Acid Desaturases from Anemone leveillei: Identification of an Acyl-Coenzyme A C20 {Delta}5-Desaturase Responsible for the Synthesis of Sciadonic Acid
Plant Physiology, May 1, 2007; 144(1): 455 - 467.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Kaewsuwan, E. B. Cahoon, P.-F. Perroud, C. Wiwat, N. Panvisavas, R. S. Quatrano, D. J. Cove, and N. Bunyapraphatsara
Identification and Functional Characterization of the Moss Physcomitrella patens {Delta}5-Desaturase Gene Involved in Arachidonic and Eicosapentaenoic Acid Biosynthesis
J. Biol. Chem., August 4, 2006; 281(31): 21988 - 21997.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Kajikawa, K. T. Yamato, Y. Kohzu, S.-i. Shoji, K. Matsui, Y. Tanaka, Y. Sakai, and H. Fukuzawa
A Front-end Desaturase from Chlamydomonas reinhardtii Produces Pinolenic and Coniferonic Acids by {omega}13 Desaturation in Methylotrophic Yeast and Tobacco
Plant Cell Physiol., January 1, 2006; 47(1): 64 - 73.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Mietkiewska, E. M. Giblin, S. Wang, D. L. Barton, J. Dirpaul, J. M. Brost, V. Katavic, and D. C. Taylor
Seed-Specific Heterologous Expression of a Nasturtium FAE Gene in Arabidopsis Results in a Dramatic Increase in the Proportion of Erucic Acid
Plant Physiology, September 1, 2004; 136(1): 2665 - 2675.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. B. Cahoon and A. J. Kinney
Dimorphecolic Acid Is Synthesized by the Coordinate Activities of Two Divergent {Delta}12-Oleic Acid Desaturases
J. Biol. Chem., March 26, 2004; 279(13): 12495 - 12502.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. M. Herman, R. M. Helm, R. Jung, and A. J. Kinney
Genetic Modification Removes an Immunodominant Allergen from Soybean
Plant Physiology, May 1, 2003; 132(1): 36 - 43.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Moon, M. A. Smith, and L. Kunst
A Condensing Enzyme from the Seeds of Lesquerella fendleri That Specifically Elongates Hydroxy Fatty Acids
Plant Physiology, December 1, 2001; 127(4): 1635 - 1643.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2000 by the American Society of Plant Biologists