|
Plant Physiol, September 2000, Vol. 124, pp. 253-264
Isolation of a cDNA Encoding a Granule-Bound 152-Kilodalton
Starch-Branching Enzyme in Wheat1
Monica
Båga,
Ramesh B.
Nair,
Anne
Repellin,
Graham J.
Scoles, and
Ravindra N.
Chibbar*
Plant Biotechnology Institute, National Research Council of Canada,
110 Gymnasium Place, Saskatoon, Saskatchewan, Canada S7N 0W9 (M.B.,
R.B.N., A.R., R.N.C.); and Department of Plant Science, University of
Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, Canada S7N 5A8
(G.J.S.)
Screening of a wheat (Triticum aestivum) cDNA
library for starch-branching enzyme I (SBEI) genes combined with
5'-rapid amplification of cDNA ends resulted in isolation of a 4,563-bp
composite cDNA, Sbe1c. Based on sequence alignment to
characterized SBEI cDNA clones isolated from plants, the SBEIc
predicted from the cDNA sequence was produced with a transit peptide
directing the polypeptide into plastids. Furthermore, the predicted
mature form of SBEIc was much larger (152 kD) than previously
characterized plant SBEI (80-100 kD) and contained a partial
duplication of SBEI sequences. The first SBEI domain showed high amino
acid similarity to a 74-kD wheat SBEI-like protein that is inactive as
a branching enzyme when expressed in Escherichia coli.
The second SBEI domain on SBEIc was identical in sequence to a
functional 87-kD SBEI produced in the wheat endosperm. Immunoblot
analysis of proteins produced in developing wheat kernels demonstrated
that the 152-kD SBEIc was, in contrast to the 87- to 88-kD SBEI,
preferentially associated with the starch granules. Proteins similar in
size and recognized by wheat SBEI antibodies were also present in
Triticum monococcum, Triticum tauschii,
and Triticum turgidum subsp. durum.
1
This work was supported by the National Research
Council of Canada (NRCC no. 43786).
*
Corresponding author; e-mail ravi.chibbar{at}nrc.ca; fax
306-975-4839.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
I. J. Tetlow, M. K. Morell, and M. J. Emes
Recent developments in understanding the regulation of starch metabolism in higher plants
J. Exp. Bot.,
October 1, 2004;
55(406):
2131 - 2145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. J. Tetlow, R. Wait, Z. Lu, R. Akkasaeng, C. G. Bowsher, S. Esposito, B. Kosar-Hashemi, M. K. Morell, and M. J. Emes
Protein Phosphorylation in Amyloplasts Regulates Starch Branching Enzyme Activity and Protein-Protein Interactions
PLANT CELL,
March 1, 2004;
16(3):
694 - 708.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Mizuno, K. Higuchi, T. Sakamoto, H. Nakanishi, S. Mori, and N. K. Nishizawa
Three Nicotianamine Synthase Genes Isolated from Maize Are Differentially Regulated by Iron Nutritional Status
Plant Physiology,
August 1, 2003;
132(4):
1989 - 1997.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Hamada, H. Ito, S. Hiraga, K. Inagaki, K. Nozaki, N. Isono, Y. Yoshimoto, Y. Takeda, and H. Matsui
Differential Characteristics and Subcellular Localization of Two Starch-branching Enzyme Isoforms Encoded by a Single Gene in Phaseolus vulgaris L.
J. Biol. Chem.,
May 3, 2002;
277(19):
16538 - 16546.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Rahman, A. Regina, Z. Li, Y. Mukai, M. Yamamoto, B. Kosar-Hashemi, S. Abrahams, and M. K. Morell
Comparison of Starch-Branching Enzyme Genes Reveals Evolutionary Relationships Among Isoforms. Characterization of a Gene for Starch-Branching Enzyme IIa from the Wheat D Genome Donor Aegilops tauschii
Plant Physiology,
March 1, 2001;
125(3):
1314 - 1324.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Peng, M. Gao, M. Båga, P. Hucl, and R. N. Chibbar
Starch-Branching Enzymes Preferentially Associated with A-Type Starch Granules in Wheat Endosperm
Plant Physiology,
September 1, 2000;
124(1):
265 - 272.
[Abstract]
[Full Text]
|
|
|
|
|
