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Plant Physiol, September 2000, Vol. 124, pp. 285-296
Polyphenol Oxidase from Hybrid Poplar. Cloning and Expression in
Response to Wounding and Herbivory1
C. Peter
Constabel,*
Lynn
Yip,
Joseph J.
Patton, and
Mary E.
Christopher
Department of Biological Sciences, University of Alberta,
Edmonton, Alberta, Canada TGG 2E9
The inducible expression of polyphenol oxidase (PPO), a presumed
antiherbivore enzyme, was examined in hybrid poplar (Populus trichocarpa × Populus deltoides).
Following mechanical wounding simulating insect damage, PPO activity
increased dramatically in wounded and unwounded leaves on wounded
plants beginning at 24 and 48 h, respectively. A hybrid poplar PPO
cDNA was isolated and its nucleotide sequence determined. On northern
blots, PPO transcripts were detected within 8 h of wounding, and
reached peak levels at 16 and 24 h in wounded and unwounded
leaves, respectively. Methyl jasmonate spray and feeding by forest tent
caterpillar also induced PPO expression. The induction of PPO was
strongest in the youngest four leaves, which were generally avoided by
caterpillars in free feeding experiments. This wound- and
herbivore-induced expression of PPO in hybrid poplar supports the
defensive role of this protein against insect pests.
1
This work was funded by a research grant from
the Natural Sciences and Engineering Research Council of Canada (to
C.P.C.).
*
Corresponding author; e-mail peter.constabel{at}ualberta.ca; fax
780-492-9234.
© 2000 American Society of Plant Physiologists
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