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Plant Physiol, September 2000, Vol. 124, pp. 297-312
Post-Transcriptional Maturation of the S Receptor Kinase of
Brassica Correlates with Co-Expression of the
S-Locus Glycoprotein in the Stigmas of Two
Brassica Strains and in Transgenic Tobacco
Plants1
Ram
Dixit,
Mikhail E.
Nasrallah, and
June B.
Nasrallah*
Department of Plant Biology, Cornell University, Ithaca, New York
14853
The S-locus-encoded S receptor kinase (SRK) is an
intrinsic plasma membrane protein that is viewed as the primary stigma
determinant of specificity in the self-incompatibility response of
Brassica spp. We analyzed two self-compatible
mutant strains that express low levels of the S-locus
glycoprotein (SLG), a cell wall-localized protein also encoded at the
S locus that is coordinately expressed with SRK. We
found that mutant stigmas synthesized wild-type levels of
SRK transcripts but failed to produce SRK protein at any
of the developmental stages analyzed. Furthermore, SRK was shown to
form aberrant high-molecular mass aggregates when expressed alone in
transgenic tobacco (Nicotiana tabacum) plants. This
aggregation was prevented in tobacco plants that co-expressed SRK and
SLG, but not in tobacco plants that co-expressed SRK and SLR1, an
SLG-related secreted protein not encoded at the S locus.
In analyses of protein extracts under reducing and non-reducing
conditions, evidence of intermolecular association was obtained only
for SLG, a fraction of which formed disulfide-linked oligomers and was
membrane associated. The data indicate that, at least in plants
carrying the S haplotypes we analyzed, SRK is an
inherently unstable protein and that SLG facilitates its accumulation
to physiologically relevant levels in Brassica
stigmas.
1
This work was supported by the National
Institutes of Health (grant no. GM57527) and National Science
Foundation (grant no. IBN-9631921).
*
Corresponding author; e-mail jbn2{at}cornell.edu; fax
607-255-5407.
© 2000 American Society of Plant Physiologists
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