Plant Physiol, September 2000, Vol. 124, pp. 313-320
Characterization of Protein and Transcript Levels of the
Chaperonin Containing Tailless Complex Protein-1 and Tubulin during
Light-Regulated Growth of Oat Seedlings1
Michael
Moser,
Eberhard
Schäfer, and
Bruno
Ehmann*
Institut für Biologie II der Universität Freiburg,
Schänzlestrasse, 1 D-79104 Freiburg, Germany (M.M.,
E.S.); and Pflanzenbiotechnologie/Zentrum für Angewandte
Biowissenschaften, Sonnenstrasse 5 D-79104 Freiburg, Germany
(B.E.)
In grass seedlings the network of cortical microtubules is
reorganized during light-dependent growth of coleoptiles and
mesocotyls. We investigated the effects of light-dependent growth on
the relative steady-state levels of the mRNAs and protein levels of
-tubulin and the 
-subunit of the chaperonin containing tailless
complex protein-1 in oat (Avena sativa) coleoptiles, which
were grown in different light conditions to establish different growth
responses. The soluble pools of the -subunit of the chaperonin
containing tailless complex protein-1 and -tubulin decreased in
nonelongating coleoptiles, suggesting that the dynamics of the
light-regulated soluble pool reflect the processes occurring during
reorganization of cortical microtubules. The shifts in pool sizes are
discussed in relation to the machinery that controls the dynamic
structure of cortical microtubules in plant cells.
1
This work was supported by a graduation
fellowship from Baden-Württemberg (to M.M.) and by the Deutsch
Forschungsgemeinschaft (grant no. Scha 303-6 to E.S. and B.E.) within
the priority program "Molecular cell biology of the heat stress response."
*
Corresponding author; e-mail ehmann{at}uni-freiburg.de; fax
49-761-203-2612.
© 2000 American Society of Plant Physiologists
