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Plant Physiol, September 2000, Vol. 124, pp. 355-368
Origin and Seed Phenotype of Maize low phytic acid
1-1 and low phytic acid
2-11
Victor
Raboy,*
Paola F.
Gerbasi,
Kevin A.
Young,
Sierra D.
Stoneberg,
Suewiya G.
Pickett,
Andrew T.
Bauman,
Pushpalatha P.N.
Murthy,
William F.
Sheridan, and
David S.
Ertl
United States Department of Agriculture-Agricultural Research
Service, National Small Grain Germplasm Research Facility, P.O. Box
307, Aberdeen, Idaho 83210 (V.R., P.F.G., K.A.Y., S.D.S., S.G.P.);
Department of Chemistry, Michigan Technological University, Houghton,
Michigan 49931 (A.T.B., P.P.N.M.); Biology Department, University of
North Dakota, Grand Forks, North Dakota 58202 (W.F.S.); and Pioneer
Hi-Bred International, P.O. Box 85, Johnston, Iowa 50131 (D.S.E.)
Phytic acid (myo-inositol-1, 2, 3, 4, 5, 6-hexakisphosphate or Ins P6) typically
represents approximately 75% to 80% of maize (Zea
mays) seed total P. Here we describe the origin, inheritance, and seed phenotype of two non-lethal maize low phytic
acid mutants, lpa1-1 and lpa2-1.
The loci map to two sites on chromosome 1S. Seed phytic acid P is
reduced in these mutants by 50% to 66% but seed total P is unaltered.
The decrease in phytic acid P in mature lpa1-1 seeds is
accompanied by a corresponding increase in inorganic phosphate
(Pi). In mature lpa2-1 seed it is
accompanied by increases in Pi and at least three other
myo-inositol (Ins) phosphates (and/or their respective
enantiomers): D-Ins(1,2,4,5,6) P5;
D-Ins (1,4,5,6) P4; and
D-Ins(1,2,6) P3. In both cases the sum of seed
Pi and Ins phosphates (including phytic acid) is constant
and similar to that observed in normal seeds. In both mutants P
chemistry appears to be perturbed throughout seed development.
Homozygosity for either mutant results in a seed dry weight loss,
ranging from 4% to 23%. These results indicate that phytic acid
metabolism during seed development is not solely responsible for P
homeostasis and indicate that the phytic acid concentration typical of
a normal maize seed is not essential to seed function.
1
This work was supported in part by the
Cooperative Research and Development Agreement (grant no.
58-3K95-3-166) between Pioneer Hi-Bred International and the U.S.
Department of Agriculture-Agricultural Research Service.
*
Corresponding author; e-mail vraboy{at}uidaho.edu; fax
208-397-4165.
© 2000 American Society of Plant Physiologists
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