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Plant Physiol, September 2000, Vol. 124, pp. 451-460
Specific Combinations of Zein Genes and Genetic Backgrounds
Influence the Transcription of the Heavy-Chain Zein Genes in Maize
opaque-2 Endosperms1
Pietro
Ciceri,2
Silvana
Castelli,
Massimiliano
Lauria,
Barbara
Lazzari,
Annamaria
Genga,
Loris
Bernard,
Monica
Sturaro, and
Angelo
Viotti*
Istituto Biosintesi Vegetali, Consiglio Nazionale delle Ricerche,
Via Bassini 15, I-20133 Milano, Italy
The transcript levels of heavy-chain zein genes (zH1
and zH2) and the occurrence of the zH polypeptides in
different opaque-2 (o2) lines were
investigated by RNA-blot analyses and by sodium dodecylsulfate-polyacrylamide gel electrophoresis or two-dimensional gel electrophoresis protein fractionations. Four mutant alleles o2R, o2T, o2It, and o2-676 introgressed
into different genetic backgrounds (GBs) were
considered. The mono-dimensional gel electrophoresis zein pattern can
be either conserved or different among the various GBs
carrying the same o2 allele. Likewise, in the identical
GB carrying different o2 alleles, the
zein pattern can be either conserved or differentially affected by the
different mutant allele. Zein protein analysis of reciprocal crosses
between lines with different o2 alleles or the same
o2 showed in some case a more than additive zH pattern
in respect to the o2 parent lines. Electrophoretic mobility
shift assay approaches, with O2-binding oligonucleotide and endosperm
extracts from the above o2 lines, failed to reveal o2-specific retarded band in any of the o2 extracts. The
results suggest that the promoter of some zH1 and
zH2 contains motif(s) that can respond to factors other
than O2.
1
This work was partially supported by a grant
from Piano Nazionale Biotecnologie Vegetali, Ministero per le Politiche
Agricole (to A.V.).
2
Present address: Department of Biology,
University of California at San Diego, La Jolla, CA
92093-0116.
*
Corresponding author; e-mail aviotti{at}icm.mi.cnr.it; fax 39-2- 23699411.
© 2000 American Society of Plant Physiologists
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