|
Plant Physiol, September 2000, Vol. 124, pp. 475-483
Subcellular Localization of a High Affinity Binding Site for
D-myo-Inositol 1,4,5-Trisphosphate from
Chenopodium rubrum1
Jan
Martinec,*
Tomá
Feltl,
Chris H.
Scanlon,
Peter J.
Lumsden, and
Ivana
Machá ková
Institute of Experimental Botany, Academy of Sciences of the Czech
Republic, Rozvojová 135, 165 02 Prague 6, Czech Republic (J.M.,
T.F., I.M.); and Department of Biological Sciences, University of
Central Lancashire, Preston, PR1 2HE, United Kingdom (C.H.S.,
P.J.L.)
It is now generally accepted that a phosphoinositide cycle is
involved in the transduction of a variety of signals in plant cells. In
animal cells, the binding of D-myo-inositol
1,4,5-trisphosphate (InsP3) to a receptor located on the
endoplasmic reticulum (ER) triggers an efflux of calcium release from
the ER. Sites that bind InsP3 with high affinity and
specificity have also been described in plant cells, but their precise
intracellular locations have not been conclusively identified. In
contrast to animal cells, it has been suggested that in plants the
vacuole is the major intracellular store of calcium involved in signal
induced calcium release. The aim of this work was to determine the
intracellular localization of InsP3-binding sites obtained
from 3-week-old Chenopodium rubrum leaves. Microsomal
membranes were fractionated by sucrose density gradient centrifugation
in the presence and absence of Mg2+ and alternatively by
free-flow electrophoresis. An ER-enriched fraction was also prepared.
The following enzymes were employed as specific membrane markers:
antimycin A-insensitive NADH-cytochrome c reductase for ER, cytochrome
c oxidase for mitochondrial membrane, pyrophosphatase for tonoplast,
and 1,3- -D-glucansynthase for plasma membrane. In all
membrane separations, InsP3-binding sites were concentrated
in the fractions that were enriched with ER membranes. These data
clearly demonstrate that the previously characterized
InsP3-binding site from C. rubrum is
localized on the ER. This finding supports previous suggestions of an
alternative non-vacuolar InsP3-sensitive calcium store in
plant cells.
1
This work was supported by the Grant Agency of
the Czech Republic (grant nos. GA204/96/0599 and GA206/96/K188 to J.M.
and I.M.).
*
Corresponding author; e-mail martinec{at}ueb.cas.cz; fax
420-2-20390419.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
P. Poutrain, C. Mazars, M. Thiersault, M. Rideau, and O. Pichon
Two distinct intracellular Ca2+-release components act in opposite ways in the regulation of the auxin-dependent MIA biosynthesis in Catharanthus roseus cells
J. Exp. Bot.,
March 1, 2009;
60(4):
1387 - 1398.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Sun, H. Miwa, J. A. Downie, and G. E.D. Oldroyd
Mastoparan Activates Calcium Spiking Analogous to Nod Factor-Induced Responses in Medicago truncatula Root Hair Cells
Plant Physiology,
June 1, 2007;
144(2):
695 - 702.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. I. Pottosin and G. Schonknecht
Vacuolar calcium channels
J. Exp. Bot.,
May 1, 2007;
58(7):
1559 - 1569.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Krinke, Z. Novotna, O. Valentova, and J. Martinec
Inositol trisphosphate receptor in higher plants: is it real?
J. Exp. Bot.,
February 1, 2007;
58(3):
361 - 376.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Monteiro, Q. Liu, S. Lisboa, G. E. F. Scherer, H. Quader, and R. Malho
Phosphoinositides and phosphatidic acid regulate pollen tube growth and reorientation through modulation of [Ca2+]c and membrane secretion
J. Exp. Bot.,
June 1, 2005;
56(416):
1665 - 1674.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Zhao, K. Inoue, I. Kouno, and H. Yamamoto
Characterization of Leachianone G 2'' -Dimethylallyltransferase, a Novel Prenyl Side-Chain Elongation Enzyme for the Formation of the Lavandulyl Group of Sophoraflavanone G in Sophora flavescens Ait. Cell Suspension Cultures
Plant Physiology,
November 1, 2003;
133(3):
1306 - 1313.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Novotna, J. Martinec, B. Profotova, S. Zdarova, J.-C. Kader, and O. Valentova
In vitro distribution and characterization of membrane-associated PLD and PI-PLC in Brassica napus
J. Exp. Bot.,
February 1, 2003;
54(383):
691 - 698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Sanders, J. Pelloux, C. Brownlee, and J. F. Harper
Calcium at the Crossroads of Signaling
PLANT CELL,
May 1, 2002;
14(90001):
S401 - 417.
[Full Text]
[PDF]
|
|
|
|
|
