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Plant Physiol, September 2000, Vol. 124, pp. 7-16
Tetrad Analysis in Higher Plants. A Budding Technology
Gregory P.
Copenhaver,*
Kevin C.
Keith, and
Daphne
Preuss
Department of Molecular Genetics and Cell Biology, University of
Chicago, Chicago, Illinois 60637
Tetrad analysis, the ability to manipulate and individually study
the four products of a single meiotic event, has been critical to
understanding the mechanisms of heredity. The Arabidopsis quartet (qrt) mutation, which causes the four products of male
meiosis to remain attached, enables plant biologists to apply this
powerful tool to investigations of gamete development, cell division,
chromosome dynamics, and recombination. Here we highlight several
examples of how qrt has been used to perform tetrad
analysis and suggest additional applications including a genetic screen
for gametophytic mutants and methods for investigating gene
interactions by synthetic lethal analysis.
*
Corresponding author; e-mail
gpcopenh{at}midway.uchicago.edu; fax 773-702-9270.
© 2000 American Society of Plant Physiologists
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