|
Plant Physiol, September 2000, Vol. 124, pp. 85-94
Sugar Uptake and Transport in Rice Embryo. Expression of
Companion Cell-Specific Sucrose Transporter (OsSUT1)
Induced by Sugar and Light1
Chi-aki
Matsukura,2
Toshikazu
Saitoh,2
Toshiro
Hirose,3
Ryu
Ohsugi,
Pierdomenico
Perata, and
Junji
Yamaguchi*
Bioscience Center and Graduate School of Bioagricultural Sciences,
Nagoya University, Chikusa, Nagoya 464-8601, Japan (C.M., T.S., J.Y.);
Department of Plant Physiology, National Institute of
Agrobiological Resources, Tsukuba, Ibaraki, 305, Japan (T.H., R.O.);
and Dip. Biologia Patologia Vegetale, University of Bari, Via
Orabona 4, I-70125 Bari, Italy (P.P.)
We investigated sugar uptake and transport in rice (Oryza
sativa) embryo during grain germination. Endogenous sugar
levels, accumulation of starch granules, and gene expression of a rice sucrose transporter (OsSUT1) were examined using rice
embryos germinated with or without exogenous sugar supply. Starch
granules remarkably accumulated in the cells around vascular bundles as a consequence of the sugar taken up by the embryos, indicating that the
taken-up sugars are transiently converted into starch. In situ
detection for OsSUT1 mRNA indicated its localization in the phloem companion cells. Furthermore, northern-blot and in situ
hybridization analyses showed that OsSUT1 expression is
not detectable in embryos subjected to sugar starvation conditions, whereas its expression is enhanced by an increased endogenous sugar
level. Overall results indicate that the expression of companion cell-specific sucrose transporter, OsSUT1 is regulated
by the endogenous sugar status as well as light exposure.
1
This work was supported by Grants-in-Aid for
Scientific Research on Priority Areas (grant nos. 10170215 and
09660004) from the Ministry of Education, Science, Sports and Culture, Japan.
2
These authors contributed equally to this paper.
3
Present address: Laboratory of Rice Metabolism, Hokuriku
National Agricultural Experiment Station, 1-2-1 Inada, Joetsu,
Niigata 943-0193, Japan.
*
Corresponding author; e-mail jjyama{at}agr.nagoya-u.ac.jp; fax
81-52-789-5219.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
A. Dusotoit-Coucaud, N. Brunel, P. Kongsawadworakul, U. Viboonjun, A. Lacointe, J.-L. Julien, H. Chrestin, and S. Sakr
Sucrose importation into laticifers of Hevea brasiliensis, in relation to ethylene stimulation of latex production
Ann. Bot.,
September 1, 2009;
104(4):
635 - 647.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R.-C. Fan, C.-C. Peng, Y.-H. Xu, X.-F. Wang, Y. Li, Y. Shang, S.-Y. Du, R. Zhao, X.-Y. Zhang, L.-Y. Zhang, et al.
Apple Sucrose Transporter SUT1 and Sorbitol Transporter SOT6 Interact with Cytochrome b5 to Regulate Their Affinity for Substrate Sugars
Plant Physiology,
August 1, 2009;
150(4):
1880 - 1901.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Lalonde and W. B. Frommer
Mendel's bequest advanced the understanding of regulatory systems for controlling sugar supply to developing plant embryos
J. Exp. Bot.,
January 1, 2009;
60(1):
1 - 3.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Zhou, K. Chan, T. L. Wang, C. L. Hedley, C. E. Offler, and J. W. Patrick
Intracellular sucrose communicates metabolic demand to sucrose transporters in developing pea cotyledons
J. Exp. Bot.,
January 1, 2009;
60(1):
71 - 85.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Braun and T. L. Slewinski
Genetic Control of Carbon Partitioning in Grasses: Roles of Sucrose Transporters and Tie-dyed Loci in Phloem Loading
Plant Physiology,
January 1, 2009;
149(1):
71 - 81.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. Srivastava, S. Ganesan, I. O. Ismail, and B. G. Ayre
Functional Characterization of the Arabidopsis AtSUC2 Sucrose/H+ Symporter by Tissue-Specific Complementation Reveals an Essential Role in Phloem Loading But Not in Long-Distance Transport
Plant Physiology,
September 1, 2008;
148(1):
200 - 211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Oikawa, A. Rahman, T. Yamashita, H. Taira, and S.-i. Kidou
Virus-induced gene silencing of P23k in barley leaf reveals morphological changes involved in secondary wall formation
J. Exp. Bot.,
July 1, 2007;
58(10):
2617 - 2625.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. N. Scofield, N. Aoki, T. Hirose, M. Takano, C. L. D. Jenkins, and R. T. Furbank
The role of the sucrose transporter, OsSUT1, in germination and early seedling growth and development of rice plants
J. Exp. Bot.,
February 1, 2007;
58(3):
483 - 495.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Conde, A. Agasse, D. Glissant, R. Tavares, H. Geros, and S. Delrot
Pathways of Glucose Regulation of Monosaccharide Transport in Grape Cells
Plant Physiology,
August 1, 2006;
141(4):
1563 - 1577.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Aoki, G. N. Scofield, X.-D. Wang, C. E. Offler, J. W. Patrick, and R. T. Furbank
Pathway of Sugar Transport in Germinating Wheat Seeds
Plant Physiology,
August 1, 2006;
141(4):
1255 - 1263.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Asatsuma, C. Sawada, K. Itoh, M. Okito, A. Kitajima, and T. Mitsui
Involvement of {alpha}-Amylase I-1 in Starch Degradation in Rice Chloroplasts
Plant Cell Physiol.,
June 1, 2005;
46(6):
858 - 869.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Aoki, T. Hirose, G. N. Scofield, P. R. Whitfeld, and R. T. Furbank
The Sucrose Transporter Gene Family in Rice
Plant Cell Physiol.,
March 15, 2003;
44(3):
223 - 232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Atanassova, M. Leterrier, C. Gaillard, A. Agasse, E. Sagot, P. Coutos-Thevenot, and S. Delrot
Sugar-Regulated Expression of a Putative Hexose Transport Gene in Grape
Plant Physiology,
January 1, 2003;
131(1):
326 - 334.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Lu, O. A. Koroleva, J. F. Farrar, J. Gallagher, C. J. Pollock, and A. D. Tomos
Rubisco Small Subunit, Chlorophyll a/b-Binding Protein and Sucrose:Fructan-6-Fructosyl Transferase Gene Expression and Sugar Status in Single Barley Leaf Cells in Situ. Cell Type Specificity and Induction by Light
Plant Physiology,
November 1, 2002;
130(3):
1335 - 1348.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ishimaru, T. Hirose, N. Aoki, S. Takahashi, K. Ono, S. Yamamoto, J. Wu, S. Saji, T. Baba, M. Ugaki, et al.
Antisense Expression of a Rice Sucrose Transporter OsSUT1 in Rice (Oryza sativa L.)
Plant Cell Physiol.,
October 1, 2001;
42(10):
1181 - 1185.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
