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Plant Physiol, September 2000, Vol. 124, pp. 95-104
Analysis of the Expression of CLA1, a Gene That
Encodes the 1-Deoxyxylulose 5-Phosphate Synthase of the
2-C-Methyl-D-Erythritol-4-Phosphate Pathway in
Arabidopsis1
Juan M.
Estévez,
Araceli
Cantero,
Cynthia
Romero,
Hiroshi
Kawaide,
Luis F.
Jiménez,
Tomohisa
Kuzuyama,
Haruo
Seto,
Yuji
Kamiya, and
Patricia
León*
Departamento de Biología Molecular de Plantas, Instituto de
Biotecnología, Universidad Nacional Autónoma de
México, Avenida Universidad 2001 Chamilpa, Apdo Postal 510-3
Cuernavaca Morelos 62271, México (J.M.E., A.C., C.R., P.L.);
Frontier Research Program, Institute of Physical and Chemical Research
(RIKEN), Hirosawa 2-1, Wako-shi, Saitama, 351-0198, Japan (H.K.,
Y.K.); Laboratorio de Microscopía Electrónica, Facultad
de Ciencias, Universidad Nacional Autónoma de México,
México (L.F.J.); and Institute of Molecular and Cellular
Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan
(T.K., H.S.)
The discovery of the
2-C-methyl-D-erythritol-4-phosphate pathway
for the biosynthesis of isoprenoids raises the important question of
the nature and regulation of the enzymes involved in this pathway.
CLA1, a gene previously isolated from Arabidopsis, encodes the first enzyme of the
2-C-methyl-D-erythritol-4-phosphate pathway,
1-deoxy-D-xylulose-5-phosphate synthase. We demonstrate this enzyme activity by complementation of the cla1-1
mutant phenotype and by direct enzymatic assays. Based on mRNA and
protein expression patterns this enzyme is expressed mainly in
developing photosynthetic and non-photosynthetic tissues. The
 -glucuronidase expression pattern driven from the
CLA1 gene regulatory region supports the northern and
protein data while also showing that this gene has some level of
expression in most tissues of the plant. A mutation in the
CLA1 gene interferes with the normal development of
chloroplasts and etioplasts, but does not seem to affect amyloplast
structure. Microscopic analysis also shows a pleiotropic effect of the
CLA1 gene mutation in mesophyll tissue formation.
1
This work was funded by Consejo Nacional de
Ciencia y Tecnologia and Dirección General de Asuntos para el
Personal Académico (grant nos. 110P-N9506 and IN205697) and by
the Pew Charitable Trust.
*
Corresponding author; e-mail patricia{at}ibt.unam.mx; fax
52-73-139988.
© 2000 American Society of Plant Physiologists
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