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Plant Physiol, October 2000, Vol. 124, pp. 553-562
Hormonal Interactions in the Control of Arabidopsis Hypocotyl
Elongation1
Clare E.
Collett,
Nicholas P.
Harberd, and
Ottoline
Leyser*
Department of Biology, The Plant Laboratory, University of York,
York YO10 5YW, United Kingdom (C.E.C., O.L.); and The John Innes
Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom
(N.P.H.)
The Arabidopsis hypocotyl, together with hormone mutants and
chemical inhibitors, was used to study the role of auxin in cell elongation and its possible interactions with ethylene and gibberellin. When wild-type Arabidopsis seedlings were grown on media containing a
range of auxin concentrations, hypocotyl growth was inhibited. However,
when axr1-12 and 35S-iaaL
(which have reduced auxin response and levels, respectively) were grown
in the same conditions, auxin was able to promote hypocotyl growth. In
contrast, auxin does not promote hypocotyl growth of
axr3-1, which has phenotypes that suggest
an enhanced auxin response. These results are consistent with the
hypothesis that auxin levels in the wild-type hypocotyl are optimal for
elongation and that additional auxin is inhibitory. When ethylene
responses were reduced using either the ethylene-resistant mutant
etr1 or aminoethoxyvinylglycine, an inhibitor of
ethylene synthesis, auxin responses were unchanged, indicating that
auxin does not inhibit hypocotyl elongation through ethylene. To test for interactions between auxin and gibberellin, auxin mutants were
grown on media containing gibberellin and gibberellin mutants were
grown on media containing auxin. The responses were found to be the
same as wild-type Arabidopsis seedlings in all cases. In addition, 1 µM of the auxin transport inhibitor 1-naphthylphthalmic acid does not alter the response of wild-type seedlings to gibberellin. Double mutants were made between gibberellin and auxin mutants and the
phenotypes of these appear additive. These results indicate that auxin
and gibberellin are acting independently in hypocotyl elongation. Thus
auxin, ethylene, and gibberellin each regulate hypocotyl elongation independently.
1
This work was supported by the Biotechnology and
Biological Sciences Research Council.
*
Corresponding author; e-mail hmol1{at}york.ac.uk; fax
44-1904-434312.
© 2000 American Society of Plant Physiologists
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