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Plant Physiol, October 2000, Vol. 124, pp. 579-586
The Arabidopsis UVH1 Gene Is a Homolog of the Yeast
Repair Endonuclease RAD11
Ana Lena
Fidantsef,
David Lewis
Mitchell, and
Anne Bagg
Britt*
Section of Plant Biology, University of California, Davis,
California 95616 (A.L.F., A.B.B.); and Department of
Carcinogenesis, M.D. Anderson Cancer Research Center, University of
Texas, Smithville, Texas 78957 (D.L.M.)
Ultraviolet radiation induces DNA damage products, largely in the
form of pyrimidine dimers, that are both toxic and mutagenic. In most
organisms, including Arabidopsis, these lesions are repaired both
through a dimer-specific photoreactivation mechanism and through a less
efficient light-independent mechanism. Several mutants defective in
this "dark repair" pathway have been previously described. The
mechanism of this repair has not been elucidated, but is thought to be
homologous to the nucleotide excision repair mechanisms found in other
eukaryotes. Here we report the complementation of the Arabidopsis
uvh1 dark repair mutant with the Arabidopsis homolog of
the yeast nucleotide excision repair gene RAD1, which encodes one of the subunits of the 5'-repair endonuclease. The uvh1-2 mutant allele carries a
glycine aspartate amino acid change that has been previously
identified to produce a null allele of RAD1 in yeast.
Although Arabidopsis homologs of genes involved in nucleotide excision
repair are readily identified by searching the genomic database, it has
not been established that these homologs are actually required for dark
repair in plants. The complementation of the Arabidopsis
uvh1 mutation with the Arabidopsis RAD1
homolog clearly demonstrates that the mechanism of nucleotide excision repair is conserved among the plant, animal, and fungal kingdoms.
1
This work was supported by the U.S. Department
of Agriculture National Research Initiative Competitive Grants
Program (grant no. 98-35301-6058).
*
Corresponding author; e-mail abbritt{at}ucdavis.edu; fax
530-752-5410.
© 2000 American Society of Plant Physiologists
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