Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (35)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nakazono, M.
Right arrow Articles by Hirai, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakazono, M.
Right arrow Articles by Hirai, A.
Agricola
Right arrow Articles by Nakazono, M.
Right arrow Articles by Hirai, A.

Plant Physiol, October 2000, Vol. 124, pp. 587-598

Expression of a Gene Encoding Mitochondrial Aldehyde Dehydrogenase in Rice Increases under Submerged Conditions1

Mikio Nakazono,* Hiroyuki Tsuji, Yuhua Li, Daisuke Saisho,2 Shin-ichi Arimura, Nobuhiro Tsutsumi, and Atsushi Hirai

Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan (M.N., H.T., Y.L., D.S., S.A., N.T., A.H.); and Faculty of Landscape Architecture, Northeast Forestry University, Harbin 150040, China (Y.L.)

It is known that alcoholic fermentation is important for survival of plants under anaerobic conditions. Acetaldehyde, one of the intermediates of alcoholic fermentation, is not only reduced by alcohol dehydrogenase but also can be oxidized by aldehyde dehydrogenase (ALDH). To determine whether ALDH plays a role in anaerobic metabolism in rice (Oryza sativa L. cv Nipponbare), we characterized a cDNA clone encoding mitochondrial ALDH from rice (Aldh2a). Analysis of sub-cellular localization of ALDH2a protein using green fluorescent protein and an in vitro ALDH assay using protein extracts from Escherichia coli cells that overexpressed ALDH2a indicated that ALDH2a functions in the oxidation of acetaldehyde in mitochondria. A Southern-blot analysis indicated that mitochondrial ALDH is encoded by at least two genes in rice. We found that the Aldh2a mRNA was present at high levels in leaves of dark-grown seedlings, mature leaf sheaths, and panicles. It is interesting that expression of the rice Aldh2a gene, unlike the expression of the tobacco (Nicotiana tabacum) Aldh2a gene, was induced in rice seedlings by submergence. Experiments with ruthenium red, which is a blocker of Ca2+ fluxes in rice as well as maize (Zea mays), suggest that the induction of expression of Adh1 and Pdc1 by low oxygen stress is regulated by elevation of the cytosolic Ca2+ level. However, the induction of Aldh2a gene expression may not be controlled by the cytosolic Ca2+ level elevation. A possible involvement of ALDH2a in the submergence tolerance of rice is discussed.

1 This work was supported in part by grants-in-aid from the Ministry of Science, Education and Culture of Japan (grant nos. 10556001 to M.N. and 09556002 to A.H.) and by grants from the Program for Promotion of Basic Research Activities for Innovative Biosciences of Japan (grant no. 0007 to A.H.).

2 Present address: Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan.

* Corresponding author; e-mail anakazo{at}mail.ecc.u-tokyo.ac.jp; fax 81-3-5841-5183.

© 2000 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
S. Huang, N. L. Taylor, R. Narsai, H. Eubel, J. Whelan, and A. H. Millar
Experimental Analysis of the Rice Mitochondrial Proteome, Its Biogenesis, and Heterogeneity
Plant Physiology, February 1, 2009; 149(2): 719 - 734.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J.-H. Shin, S.-R. Kim, and G. An
Rice Aldehyde Dehydrogenase7 Is Needed for Seed Maturation and Viability
Plant Physiology, February 1, 2009; 149(2): 905 - 915.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S.-i. Arimura, M. Fujimoto, Y. Doniwa, N. Kadoya, M. Nakazono, W. Sakamoto, and N. Tsutsumi
Arabidopsis ELONGATED MITOCHONDRIA1 Is Required for Localization of DYNAMIN-RELATED PROTEIN3A to Mitochondrial Fission Sites
PLANT CELL, June 1, 2008; 20(6): 1555 - 1566.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Lasanthi-Kudahettige, L. Magneschi, E. Loreti, S. Gonzali, F. Licausi, G. Novi, O. Beretta, F. Vitulli, A. Alpi, and P. Perata
Transcript Profiling of the Anoxic Rice Coleoptile
Plant Physiology, May 1, 2007; 144(1): 218 - 231.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Fujiwara, K. Umemura, T. Kawasaki, and K. Shimamoto
Proteomics of Rac GTPase Signaling Reveals Its Predominant Role in Elicitor-Induced Defense Response of Cultured Rice Cells
Plant Physiology, February 1, 2006; 140(2): 734 - 745.
[Abstract] [Full Text] [PDF]

Home page
 Drug Metab. Dispos.Home page
N. Lassen, T. Estey, R. L. Tanguay, A. Pappa, M. J. Reimers, and V. Vasiliou
MOLECULAR CLONING, BACULOVIRUS EXPRESSION, AND TISSUE DISTRIBUTION OF THE ZEBRAFISH ALDEHYDE DEHYDROGENASE 2
Drug Metab. Dispos., May 1, 2005; 33(5): 649 - 656.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. B. Nair, K. L. Bastress, M. O. Ruegger, J. W. Denault, and C. Chapple
The Arabidopsis thaliana REDUCED EPIDERMAL FLUORESCENCE1 Gene Encodes an Aldehyde Dehydrogenase Involved in Ferulic Acid and Sinapic Acid Biosynthesis
PLANT CELL, February 1, 2004; 16(2): 544 - 554.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
O. Kursteiner, I. Dupuis, and C. Kuhlemeier
The Pyruvate decarboxylase1 Gene of Arabidopsis Is Required during Anoxia But Not Other Environmental Stresses
Plant Physiology, June 1, 2003; 132(2): 968 - 978.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Liu and P. S. Schnable
Functional Specialization of Maize Mitochondrial Aldehyde Dehydrogenases
Plant Physiology, December 1, 2002; 130(4): 1657 - 1674.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2000 by the American Society of Plant Biologists