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Plant Physiol, October 2000, Vol. 124, pp. 599-608
Identification of a New Glucosinolate-Rich Cell Type in
Arabidopsis Flower Stalk
Olga A.
Koroleva,*
Andrew
Davies,
Rosalia
Deeken,
Michael R.
Thorpe,
A. Deri
Tomos, and
Rainer
Hedrich
School of Biological Sciences, University of Wales, Bangor LL57
2UW, United Kingdom (O.A.K., A.D., A.D.T.); Julius von Sachs Institut
fuer Biowissenschaften, Julius von Sachs Platz 2, D-97082 Wuerzburg,
Germany (R.D., R.H.); and HortResearch, Ruakura, Box 3123, Hamilton,
New Zealand (M.R.T.)
Distribution of K, Ca, Cl, S, and P in freeze-dried sections of
Arabidopsis flower stalk was analyzed by energy dispersive x-ray
imaging. Concentrations of these elements in different cell types were
quantified by microanalysis of single-cell samples and phloem exudates.
Results showed a differential pattern of distribution for all five
elements. K concentration was found to be highest in the parenchymatous
tissue around vascular bundles. Ca and Cl were present mainly in the
central part of the flower stalk. P was largely located in the bundles
and in the parenchyma surrounding them. S signal was extraordinary high
in groups of cells (S-cells) situated between the phloem of every
vascular bundle and the endodermis. Enzymatic hydrolysis by
thioglucosidase of cell sap collected from S-cells using a glass
microcapillary resulted in the release of glucose, indicating that
these cells contain glucosinolates at high (> 100 mM)
concentration, which is consistent with the concentration of S (> 200 mM) estimated by x-ray analysis of cell sap samples. Since
their position outside of the phloem is ideally suited for protecting
the long-distance transport system from feeding insects, the possible
roles of these cells as components of a plant defense system are discussed.
*
Corresponding author; e-mail o.koroleva{at}bangor.ac.uk; fax
44-1248-370731.
© 2000 American Society of Plant Physiologists
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