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Plant Physiol, October 2000, Vol. 124, pp. 615-626
Characterization of Arabidopsis Acid Phosphatase Promoter and
Regulation of Acid Phosphatase Expression
Shoshan
Haran,
Sithes
Logendra,
Mirjana
Seskar,
Margarita
Bratanova, and
Ilya
Raskin*
Biotech Center, Foran Hall, Cook College, Rutgers University, New
Brunswick, New Jersey 08901-8520
The expression and secretion of acid phosphatase (APase) was
investigated in Indian mustard (Brassica juncea L. Czern.) plants using sensitive in vitro and activity gel assays.
Phosphorus (P) starvation induced two APases in Indian mustard roots,
only one of which was secreted. Northern-blot analysis indicated
transcriptional regulation of APase expression. Polymerase chain
reaction and Southern-blot analyses revealed two APase homologs in
Indian mustard, whereas in Arabidopsis, only one APase homolog was
detected. The Arabidopsis APase promoter region was cloned and fused to
the  -glucuronidase (GUS) and green fluorescent protein (GFP)
reporter genes. GUS expression was first evident in leaves of the
P-starved Arabidopsis plants. In P-starved roots, the expression of GUS initiated in lateral root meristems followed by generalized expression throughout the root. GUS expression diminished with the addition of P
to the medium. Expression of GFP in P-starved roots also initiated in
the lateral root meristems and the recombinant GFP with the APase
signal peptide was secreted by the roots into the medium. The APase
promoter was specifically activated by low P levels. The removal of
other essential elements or the addition of salicylic or jasmonic
acids, known inducers of gene expression, did not activate the APase
promoter. This novel APase promoter may be used as a plant-inducible
gene expression system for the production of recombinant proteins and
as a tool to study P metabolism in plants.
*
Corresponding author; e-mail raskin{at}aesop.rutgers.edu; fax
732-932-6535.
© 2000 American Society of Plant Physiologists
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