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Plant Physiol, October 2000, Vol. 124, pp. 703-714

Redox-Regulated RNA Helicase Expression1

Sonya L. Kujat and George W. Owttrim*

Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9

In photosynthetic organisms it is becoming increasingly evident that light-driven shifts in redox potential act as a sensor that initiates alterations in gene expression at both the level of transcription and translation. This report provides evidence that the expression of a cyanobacterial RNA helicase gene, crhR, is controlled at the level of transcription and mRNA stability by a complex series of interacting mechanisms that are redox regulated. Transcript accumulation correlates with reduction of the electron transport chain between QA in photosystem II and QO in cyt b6f, when Synechocystis sp. strain PCC 6803 is cultured photoautotrophically or photomixotrophically and subjected to darkness and/or electron transport inhibitors or illumination that preferentially excites photosystem II. crhR mRNA stability is also regulated by a redox responsive mechanism, which differs from that affecting accumulation and does not involve signaling initiated by photoreceptors. The data are most consistent with plastoquinol/cyt b6f interaction as the sensor initiating a signal transduction cascade resulting in accumulation of the crhR transcript. Functionally, CrhR RNA unwinding could act as a linker between redox regulated transcription and translation. The potential for translational regulation of redox-induced gene expression through RNA helicase-catalyzed modulation of RNA secondary structure is discussed.

1 This work was supported in part by a grant from the Natural Sciences and Engineering Research Council of Canada (to G.W.O.).

* Corresponding author; e-mail g.owttrim{at}ualberta.ca; fax 780-492-9234.

© 2000 American Society of Plant Physiologists


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