|
Plant Physiol, October 2000, Vol. 124, pp. 715-724
Differential Subcellular Localization and Expression of ATP
Sulfurylase and 5'-Adenylylsulfate Reductase during Ontogenesis
of Arabidopsis Leaves Indicates That Cytosolic and Plastid Forms of ATP
Sulfurylase May Have Specialized Functions1
Carmen
Rotte2 and
Thomas
Leustek*
Biotechnology Center for Agriculture and the Environment, Rutgers
University, New Brunswick, New Jersey 08901-8520
ATP sulfurylase and 5'-adenylylsulfate (APS) reductase catalyze two
reactions in the sulfate assimilation pathway. Cell fractionation of
Arabidopsis leaves revealed that ATP sulfurylase isoenzymes exist in
the chloroplast and the cytosol, whereas APS reductase is localized
exclusively in chloroplasts. During development of Arabidopsis plants
the total activity of ATP sulfurylase and APS reductase declines by
3-fold in leaves. The decline in APS reductase can be attributed to a
reduction of enzyme during aging of individual leaves, the highest
activity occurring in the youngest leaves and the lowest in fully
expanded leaves. By contrast, total ATP sulfurylase activity declines
proportionally in all the leaves. The distinct behavior of ATP
sulfurylase can be attributed to reciprocal expression of the
chloroplast and cytosolic isoenzymes. The chloroplast form,
representing the more abundant isoenzyme, declines in parallel with APS
reductase during aging; however, the cytosolic form increases over the
same period. In total, the results suggest that cytosolic ATP
sulfurylase plays a specialized function that is probably unrelated to
sulfate reduction. A plausible function could be in generating APS for
sulfation reactions.
1
This work was supported by the National Science
Foundation (grant nos. IBN-9601146 and IBN-9817594) and by the
Studienstiftung des deutschen Volkes (to C.R.). The work was carried
out in part as a Diplomarbeit Thesis from the Carl von Ossietzky
Universität Oldenburg, Germany.
2
Present address: Institut fuer Botanik III,
Heinrich-Heine Universitaet Duesseldorf, Universitaetsstrasse 1, 40225 Duesseldorf, Germany.
*
Corresponding author; e-mail leustek{at}aesop.rutgers.edu; fax
732-932-0312.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
S. G. Mugford, N. Yoshimoto, M. Reichelt, M. Wirtz, L. Hill, S. T. Mugford, Y. Nakazato, M. Noji, H. Takahashi, R. Kramell, et al.
Disruption of Adenosine-5'-Phosphosulfate Kinase in Arabidopsis Reduces Levels of Sulfated Secondary Metabolites
PLANT CELL,
March 1, 2009;
21(3):
910 - 927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. KOPRIVA
Regulation of Sulfate Assimilation in Arabidopsis and Beyond
Ann. Bot.,
April 1, 2006;
97(4):
479 - 495.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Adai, C. Johnson, S. Mlotshwa, S. Archer-Evans, V. Manocha, V. Vance, and V. Sundaresan
Computational prediction of miRNAs in Arabidopsis thaliana
Genome Res.,
January 1, 2005;
15(1):
78 - 91.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Saito
Sulfur Assimilatory Metabolism. The Long and Smelling Road
Plant Physiology,
September 1, 2004;
136(1):
2443 - 2450.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Klein and J. Papenbrock
The multi-protein family of Arabidopsis sulphotransferases and their relatives in other plant species
J. Exp. Bot.,
August 1, 2004;
55(404):
1809 - 1820.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. J. Pickering, C. Wright, B. Bubner, D. Ellis, M. W. Persans, E. Y. Yu, G. N. George, R. C. Prince, and D. E. Salt
Chemical Form and Distribution of Selenium and Sulfur in the Selenium Hyperaccumulator Astragalus bisulcatus
Plant Physiology,
March 1, 2003;
131(3):
1460 - 1467.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. G. Ravina, C.-I. Chang, G. P. Tsakraklides, J. P. McDermott, J. M. Vega, T. Leustek, C. Gotor, and J. P. Davies
The sac Mutants of Chlamydomonas reinhardtii Reveal Transcriptional and Posttranscriptional Control of Cysteine Biosynthesis
Plant Physiology,
December 1, 2002;
130(4):
2076 - 2084.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. L. Fatland, J. Ke, M. D. Anderson, W. I. Mentzen, L. W. Cui, C. C. Allred, J. L. Johnston, B. J. Nikolau, and E. S. Wurtele
Molecular Characterization of a Heteromeric ATP-Citrate Lyase That Generates Cytosolic Acetyl-Coenzyme A in Arabidopsis
Plant Physiology,
October 1, 2002;
130(2):
740 - 756.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Koprivova, M. Melzer, P. von Ballmoos, T. Mandel, C. Brunold, and S. Kopriva
Assimilatory Sulfate Reduction in C3, C3-C4, and C4 Species of Flaveria
Plant Physiology,
October 1, 2001;
127(2):
543 - 550.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
