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Plant Physiol, October 2000, Vol. 124, pp. 795-804
Direct Evidence for Requirement of Phosphatidylglycerol in
Photosystem II of Photosynthesis1
Miki
Hagio,
Zoltán
Gombos,
Zsuzsanna
Várkonyi,
Kazumori
Masamoto,
Norihiro
Sato,
Mikio
Tsuzuki, and
Hajime
Wada*
Department of Biology, Graduate School of Sciences, Kyushu
University, Ropponmatsu, Fukuoka 810-8560, Japan (M.H., Z.G., H.W.);
Institute of Plant Biology, Biological Research Center of the Hungarian
Academy of Sciences, P.O. Box 521, H-6701 Szeged, Hungary (Z.G.,
Z.V.); Biological Laboratory, Faculty of Education, Kumamoto
University, Kurokami, Kumamoto 860-0862, Japan (K.M.); and School of
Life Science, Tokyo University of Pharmacy and Life Science,
Horinouchi, Hachioji 192-0392, Japan (N.S., M.T.)
Phosphatidylglycerol (PG) is considered to play an important role
in the ordered assembly and structural maintenance of the photosynthetic apparatus in thylakoid membranes. However, its function
in photosynthesis remains poorly understood. In this study we have
identified a pgsA gene of Synechocystis
sp. PCC6803 that encodes a PG phosphate synthase involved in the
biosynthesis of PG. A disruption of the pgsA gene
allowed us to manipulate the content of PG in thylakoid membranes and
to investigate the function of PG in photosynthesis. The obtained
pgsA mutant could grow only in the medium containing PG,
and the photosynthetic activity of the pgsA mutant
dramatically decreased with a concomitant decrease of PG content in
thylakoid membranes when the cells grown in the presence of PG were
transferred to the medium without PG. This decrease of photosynthetic
activity was attributed to the decrease of photosystem (PS)II activity,
but not to the decrease in PSI activity. These findings demonstrate
that PG is essential for growth of Synechocystis sp.
PCC6803 and provide the first direct evidence that PG plays an
important role in PSII.
1
This work was supported by a Grant-in-Aid for
Scientific Research from the Ministry of Education, Science, Sports and
Culture (grant no. 12640635 to H.W.). M.H. and Z.G. were
supported by fellowships from the Japan Society for the Promotion of Science.
*
Corresponding author; e-mail wadarcb{at}mbox.nc.kyushu-u.ac.jp; fax
81-92-726-4761.
© 2000 American Society of Plant Physiologists
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