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Plant Physiol, October 2000, Vol. 124, pp. 833-844
Differential Effects of Methyl Jasmonate on the Expression of the
Early Light-Inducible Proteins and Other Light-Regulated Genes in
Barley1
Inken
Wierstra2 and
Klaus
Kloppstech*
Institut für Botanik, Universität Hannover,
Herrenhäuser Strasse 2, D-30419 Hannover, Germany
The effects of methyl jasmonate (JA-Me) on early light-inducible
protein (ELIP) expression in barley (Hordeum vulgare L. cv Apex) have been studied. Treatment of leaf segments with JA-Me induces the same symptoms as those exhibited by norflurazon bleaching, including a loss of pigments and enhanced light stress that results in
increased ELIP expression under both high- and low-light conditions. The expression of both low- and high-molecular-mass ELIP families is
considerably down-regulated by JA-Me at the transcript and protein
levels. This repression occurs despite increased photoinhibition measurable as a massive degradation of D1 protein and a delayed recovery of photosystem II activity. In JA-Me-treated leaf segments, the decrease of the photochemical efficiency of photosystem II under
high light is substantially more pronounced as compared to controls in
water. The repression of ELIP expression by JA-Me is superimposed on
the effect of the increased light stress that leads to enhanced ELIP
expression. The fact that the reduction of ELIP transcript levels is
less pronounced than those of light-harvesting complex II and small
subunit of Rubisco transcripts indicates that light stress is still
affecting gene expression in the presence of JA-Me. The
jasmonate-induced protein transcript levels that are induced by JA-Me
decline under light stress conditions.
1
This work was supported by the Deutsche
Forschungsgemeinschaft, Bonn.
2
Present address: Institut für Molekularbiologie,
Medizinische Hochschule Hannover, Carl-Neuberg-Str.1, D-30625
Hannover, Germany.
*
Corresponding author; e-mail
kloppstech{at}mbox.botanik.uni-hannover.de; fax
0049-511-762-3992.
© 2000 American Society of Plant Physiologists
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