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Plant Physiol, October 2000, Vol. 124, pp. 873-884
Effects of Iron Deficiency on the Composition of the Leaf
Apoplastic Fluid and Xylem Sap in Sugar Beet. Implications for Iron and
Carbon Transport1
Ana Flor
López-Millán,
Fermín
Morales,
Anunciación
Abadía, and
Javier
Abadía*
Departamento de Nutrición Vegetal, Estación
Experimental de Aula Dei, Consejo Superior de Investigaciones
Científicas, Apartado 202, E-50080 Zaragoza, Spain
The effects of iron deficiency on the composition of the xylem sap
and leaf apoplastic fluid have been characterized in sugar beet
(Beta vulgaris Monohil hybrid). pH was estimated from
direct measurements in apoplastic fluid and xylem sap obtained by
centrifugation and by fluorescence of leaves incubated with
5-carboxyfluorescein and fluorescein isothiocyanate-dextran. Iron
deficiency caused a slight decrease in the pH of the leaf apoplast
(from 6.3 down to 5.9) and xylem sap (from 6.0 down to 5.7) of sugar
beet. Major organic acids found in leaf apoplastic fluid and xylem sap
were malate and citrate. Total organic acid concentration in control plants was 4.3 mM in apoplastic fluid and 9.4 mM in xylem sap and increased to 12.2 and 50.4 mM, respectively, in iron-deficient plants. Inorganic
cation and anion concentrations also changed with iron deficiency both
in apoplastic fluid and xylem sap. Iron decreased with iron deficiency
from 5.5 to 2.5 µM in apoplastic fluid and xylem sap.
Major predicted iron species in both compartments were
[FeCitOH] 1 in the controls and
[FeCit2]3 in the iron-deficient plants.
Data suggest the existence of an influx of organic acids from the roots
to the leaves via xylem, probably associated to an anaplerotic carbon
dioxide fixation by roots.
1
This work was supported by the Comisión
Interministerial de Ciencia y Tecnología (grant no. AGR97-1177
to A.A.), the Dirección General de Investigación
Científica y Técnica (grant no. PB97-1176 to J.A.), and
the Commission of European Communities (grant nos. AIR3-CT94-1973 and
PL971176 to J.A.). A.F.L.-M. and F.M. were supported by a pre-doctoral
fellowship and a scientist research contract from the Spanish Ministry
of Science and Education, respectively.
*
Corresponding author; e-mail jabadia{at}eead.csic.es; fax
34-976-575620.
© 2000 American Society of Plant Physiologists
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