Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (80)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Klarzynski, O.
Right arrow Articles by Fritig, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Klarzynski, O.
Right arrow Articles by Fritig, B.
Agricola
Right arrow Articles by Klarzynski, O.
Right arrow Articles by Fritig, B.

Plant Physiol, November 2000, Vol. 124, pp. 1027-1038

Linear beta -1,3 Glucans Are Elicitors of Defense Responses in Tobacco

Olivier Klarzynski,1 Bertrand Plesse,1 Jean-Marie Joubert, Jean-Claude Yvin, Marguerite Kopp, Bernard Kloareg, and Bernard Fritig*

Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, Université Louis Pasteur, F-67084 Strasbourg cedex, France (O.K., B.P., M.K., B.F.); and Unité Mixte de Recherche 1931 (Centre National de la Recherche Scientifique-Goëmar), Station Biologique de Roscoff, F-29682 Roscoff, France (J.-M.J., J.-C.Y., B.K.)

Laminarin, a linear beta -1,3 glucan (mean degree of polymerization of 33) was extracted and purified from the brown alga Laminaria digitata. Its elicitor activity on tobacco (Nicotiana tabacum) was compared to that of oligogalacturonides with a mean degree of polymerization of 10. The two oligosaccharides were perceived by suspension-cultured cells as distinct chemical stimuli but triggered a similar and broad spectrum of defense responses. A dose of 200 µg mL-1 laminarin or oligogalacturonides induced within a few minutes a 1.9-pH-units alkalinization of the extracellular medium and a transient release of H2O2. After a few hours, a strong stimulation of Phe ammonia-lyase, caffeic acid O-methyltransferase, and lipoxygenase activities occurred, as well as accumulation of salicylic acid. Neither of the two oligosaccharides induced tissue damage or cell death nor did they induce accumulation of the typical tobacco phytoalexin capsidiol, in contrast with the effects of the proteinaceous elicitor beta -megaspermin. Structure activity studies with laminarin, laminarin oligomers, high molecular weight beta -1,3-1,6 glucans from fungal cell walls, and the beta -1,6-1,3 heptaglucan showed that the elicitor effects observed in tobacco with beta -glucans are specific to linear beta -1,3 linkages, with laminaripentaose being the smallest elicitor-active structure. In accordance with its strong stimulating effect on defense responses in tobacco cells, infiltration of 200 µg mL-1 laminarin in tobacco leaves triggered accumulation within 48 h of the four families of antimicrobial pathogenesis-related proteins investigated. Challenge of the laminarin-infiltrated leaves 5 d after treatment with the soft rot pathogen Erwinia carotovora subsp. carotovora resulted in a strong reduction of the infection when compared with water-treated leaves.

1 These authors contributed equally to the paper.

* Corresponding author; e-mail bernard.fritig{at}ibmp-ulp.u-strasbg.fr; fax 33-3-88-61-44-42.

© 2000 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
T. Shinya, I. Galis, T. Narisawa, M. Sasaki, H. Fukuda, H. Matsuoka, M. Saito, and K. Matsuoka
Comprehensive Analysis of Glucan Elicitor-Regulated Gene Expression in Tobacco BY-2 Cells Reveals a Novel MYB Transcription Factor Involved in the Regulation of Phenylpropanoid Metabolism
Plant Cell Physiol., October 1, 2007; 48(10): 1404 - 1413.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Aziz, A. Gauthier, A. Bezier, B. Poinssot, J.-M. Joubert, A. Pugin, A. Heyraud, and F. Baillieul
Elicitor and resistance-inducing activities of {beta}-1,4 cellodextrins in grapevine, comparison with {beta}-1,3 glucans and {alpha}-1,4 oligogalacturonides
J. Exp. Bot., April 1, 2007; 58(6): 1463 - 1472.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
A. H. Lacchini, A. J. Davies, D. Mackintosh, and A. J. Walker
{beta}-1, 3-glucan modulates PKC signalling in Lymnaea stagnalis defence cells: a role for PKC in H2O2 production and downstream ERK activation
J. Exp. Biol., December 15, 2006; 209(24): 4829 - 4840.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
R. Menard, P. de Ruffray, B. Fritig, J.-C. Yvin, and S. Kauffmann
Defense and Resistance-inducing Activities in Tobacco of the Sulfated {beta}-1,3 glucan PS3 and its Synergistic Activities with the Unsulfated Molecule
Plant Cell Physiol., December 1, 2005; 46(12): 1964 - 1972.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
C. Veneault-Fourrey, R. Lauge, and T. Langin
Nonpathogenic Strains of Colletotrichum lindemuthianum Trigger Progressive Bean Defense Responses during Appressorium-Mediated Penetration
Appl. Envir. Microbiol., August 1, 2005; 71(8): 4761 - 4770.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. Menard, S. Alban, P. de Ruffray, F. Jamois, G. Franz, B. Fritig, J.-C. Yvin, and S. Kauffmann
{beta}-1,3 Glucan Sulfate, but Not {beta}-1,3 Glucan, Induces the Salicylic Acid Signaling Pathway in Tobacco and Arabidopsis
PLANT CELL, November 1, 2004; 16(11): 3020 - 3032.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Fliegmann, A. Mithofer, G. Wanner, and J. Ebel
An Ancient Enzyme Domain Hidden in the Putative {beta}-Glucan Elicitor Receptor of Soybean May Play an Active Part in the Perception of Pathogen-associated Molecular Patterns during Broad Host Resistance
J. Biol. Chem., January 9, 2004; 279(2): 1132 - 1140.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
L. Polonelli, W. Magliani, S. Conti, L. Bracci, L. Lozzi, P. Neri, D. Adriani, F. De Bernardis, and A. Cassone
Therapeutic Activity of an Engineered Synthetic Killer Antiidiotypic Antibody Fragment against Experimental Mucosal and Systemic Candidiasis
Infect. Immun., November 1, 2003; 71(11): 6205 - 6212.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. Lecourieux, C. Mazars, N. Pauly, R. Ranjeva, and A. Pugin
Analysis and Effects of Cytosolic Free Calcium Increases in Response to Elicitors in Nicotiana plumbaginifolia Cells
PLANT CELL, October 1, 2002; 14(10): 2627 - 2641.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. H.H. Borner, D. J. Sherrier, T. J. Stevens, I. T. Arkin, and P. Dupree
Prediction of Glycosylphosphatidylinositol-Anchored Proteins in Arabidopsis. A Genomic Analysis
Plant Physiology, June 1, 2002; 129(2): 486 - 499.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2000 by the American Society of Plant Biologists