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Plant Physiol, November 2000, Vol. 124, pp. 1105-1120
A Genomics Approach to the Comprehensive Analysis of the
Glutathione S-Transferase Gene Family in Soybean and
Maize
Brian
McGonigle,
Sharon J.
Keeler,
Sze-Mei Cindy
Lau,
Mary
K.
Koeppe, and
Daniel P.
O'Keefe*
Nutrition and Health (B.M.), Central Research and Development
(S.J.K., S.-M.C.L., D.P.O.), and Crop Protection (M.K.K.), E.I. du Pont
de Nemours and Company, Experimental Station, P.O. Box 80328, Wilmington, Delaware 19880-0328
By BLAST searching a large expressed sequence tag database for
glutathione S-transferase (GST) sequences we have
identified 25 soybean (Glycine max) and 42 maize
(Zea mays) clones and obtained accurate full-length GST
sequences. These clones probably represent the majority of members of
the GST multigene family in these species. Plant GSTs are divided
according to sequence similarity into three categories: types I, II,
and III. Among these GSTs only the active site serine, as well as
another serine and arginine in or near the "G-site" are conserved
throughout. Type III GSTs have four conserved sequence patches mapping
to distinct structural features. Expression analysis reveals the
distribution of GSTs in different tissues and treatments: Maize GSTI is
overall the most highly expressed in maize, whereas the previously
unknown GmGST 8 is most abundant in soybean. Using DNA microarray
analysis we observed increased expression among the type III GSTs after
inducer treatment of maize shoots, with different genes responding to
different treatments. Protein activity for a subset of GSTs varied
widely with seven substrates, and any GST exhibiting greater than
marginal activity with chloro-2,4 dinitrobenzene activity also
exhibited significant activity with all other substrates, suggesting
broad individual enzyme substrate specificity.
*
Corresponding author; e-mail
daniel.p.okeefe{at}usa.DuPont.com; fax 302-695-1374.
© 2000 American Society of Plant Physiologists
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