|
Plant Physiol, November 2000, Vol. 124, pp. 1229-1238
Overexpression of Auxin-Binding Protein Enhances the Sensitivity
of Guard Cells to Auxin1
James M.
Bauly,2*
Ian M.
Sealy,
Heather
Macdonald,
Jane
Brearley,
Swenja
Dröge,
Stefan
Hillmer,
David
G.
Robinson,
Michael A.
Venis,
Michael R.
Blatt,
Colin M.
Lazarus, and
Richard M.
Napier
Horticulture Research International, Wellesbourne, Warwick CV35
9EF, United Kingdom (J.M.B., M.A.V., R.M.N.); School of Biological
Sciences, University of Bristol, Bristol BS8 1UG, United Kingdom
(I.M.S., C.M.L.); Department of Life Sciences, University of the West
of England, Bristol BS16 1QY, United Kingdom (H.M.); Laboratory of
Plant Physiology and Biophysics, Imperial College of Science,
Technology and Medicine at Wye, Wye, Kent TN25 5AH, United Kingdom
(J.B., M.R.B.); and Albrecht-von-Haller Insitut für
Pflanzenwissensschaften, Abteilung Strukturelle Zellphysiologie
Universität Göttingen, Untere Karspüle 2, 37073 Göttingen, Germany (S.D., S.H., D.G.R.)
To explore the role of auxin-binding protein (ABP1) in planta, a
number of transgenic tobacco (Nicotiana tabacum) lines
were generated. The wild-type KDEL endoplasmic reticulum targeting signal was mutated to HDEL, another common retention sequence in
plants, and to KEQL or KDELGL to compromise its activity. The auxin-binding kinetics of these forms of ABP1 were found to be similar
to those of ABP1 purified from maize (Zea mays). To test for a physiological response mediated by auxin, intact guard cells of
the transgenic plants were impaled with double-barreled
microelectrodes, and auxin-dependent changes in K+ currents
were recorded under voltage clamp. Exogenous auxin affected inwardly
and outwardly rectifying K+ currents in a dose-dependent
manner. Auxin sensitivity was markedly enhanced in all plants
overexpressing ABP1, irrespective of the form present. Immunogold
electron microscopy was used to investigate the localization of ABP1 in
the transgenic plants. All forms were detected in the endoplasmic
reticulum and the KEQL and KDELGL forms passed further across the Golgi
stacks than KDEL and HDEL forms. However, neither electron microscopy
nor silver-enhanced immunogold epipolarization microscopy revealed
differences in cell surface ABP1 abundance for any of the plants,
including control plants, which indicated that overexpression of ABP1
alone was sufficient to confer increased sensitivity to added auxin.
Jones et al. ([1998] Science 282: 1114-1117) found increased cell
expansion in transgenic plants overexpressing wild-type ABP1. Single
cell recordings extend this observation, with the demonstration that the auxin sensitivity of guard cell K+ currents is
mediated, at least in part, by ABP1.
1
This work was jointly funded by the
Biotechnology and Biological Science Research Council, the Gatsby
Charitable Foundation, the Science and Engineering Research Council,
and the EC.
2
Present address: Institut des Sciences
Végétales, Centre National de la Recherche Scientifique,
F-91198 Gif-sur-Yvette cedex, France.
*
Corresponding author; e-mail James.Bauly{at}isv.cnrs-gif.fr; fax
33-1-69-82-36-95.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
N. Braun, J. Wyrzykowska, P. Muller, K. David, D. Couch, C. Perrot-Rechenmann, and A. J. Fleming
Conditional Repression of AUXIN BINDING PROTEIN1 Reveals That It Coordinates Cell Division and Cell Expansion during Postembryonic Shoot Development in Arabidopsis and Tobacco
PLANT CELL,
October 1, 2008;
20(10):
2746 - 2762.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-W. Hou, Y.-T. Zhou, K.-N. Mwange, W.-F. Li, X.-Q. He, and K.-M. Cui
ABP1 expression regulated by IAA and ABA is associated with the cambium periodicity in Eucommia ulmoides Oliv.
J. Exp. Bot.,
November 1, 2006;
57(14):
3857 - 3867.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Laporte, G. Vetter, A.-M. Loudes, D. G. Robinson, S. Hillmer, C. Stussi-Garaud, and C. Ritzenthaler
Involvement of the Secretory Pathway and the Cytoskeleton in Intracellular Targeting and Tubule Assembly of Grapevine fanleaf virus Movement Protein in Tobacco BY-2 Cells
PLANT CELL,
September 1, 2003;
15(9):
2058 - 2075.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ko, Y. Tekoah, P. M. Rudd, D. J. Harvey, R. A. Dwek, S. Spitsin, C. A. Hanlon, C. Rupprecht, B. Dietzschold, M. Golovkin, et al.
Function and glycosylation of plant-derived antiviral monoclonal antibody
PNAS,
June 24, 2003;
100(13):
8013 - 8018.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-F. Chen, M. D. Randlett, J. L. Findell, and G. E. Schaller
Localization of the Ethylene Receptor ETR1 to the Endoplasmic Reticulum of Arabidopsis
J. Biol. Chem.,
May 24, 2002;
277(22):
19861 - 19866.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kepinski and O. Leyser
Ubiquitination and Auxin Signaling: A Degrading Story
PLANT CELL,
May 1, 2002;
14(90001):
S81 - 95.
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Phillipson, P. Pimpl, L. L. P. daSilva, A. J. Crofts, J. P. Taylor, A. Movafeghi, D. G. Robinson, and J. Denecke
Secretory Bulk Flow of Soluble Proteins Is Efficient and COPII Dependent
PLANT CELL,
September 1, 2001;
13(9):
2005 - 2020.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Tassoni, R. M. Napier, M. Franceschetti, M. A. Venis, and N. Bagni
Spermidine-Binding Proteins. Purification and Expression Analysis in Maize
Plant Physiology,
April 1, 2002;
128(4):
1303 - 1312.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
