|
Plant Physiol, November 2000, Vol. 124, pp. 1349-1362
Permeability and Channel-Mediated Transport of Boric Acid
across Membrane Vesicles Isolated from Squash
Roots1
Christos
Dordas,2*
Maarten J.
Chrispeels, and
Patrick H.
Brown
Department of Pomology, University of California, One Shields
Avenue, Davis, California 95616 (C.D., P.H.B.); and
Department of Biology, University of California, 9500 Gilman Drive, La Jolla, California 92093-0116 (M.J.C.)
Boron is an essential micronutrient for plant growth and
the boron content of plants differs greatly, but the mechanism(s) of
its uptake into cells is not known. Boron is present in the soil
solution as boric acid and it is in this form that it enters the roots.
We determined the boron permeability coefficient of purified plasma
membrane vesicles obtained from squash (Cucurbita pepo)
roots and found it to be 3 × 10 7 ±1.4 × 108 cm s1, six times higher than the
permeability of microsomal vesicles. Boric acid permeation of the
plasma membrane vesicles was partially inhibited (30%-39%) by
mercuric chloride and phloretin, a non-specific channel blocker. The
inhibition by mercuric chloride was readily reversible by
2-mercaptoethanol. The energy of activation for boron transport into
the plasma membrane vesicles was 10.2 kcal mol1. Together
these data indicate that boron enters plant cells in part by passive
diffusion through the lipid bilayer of the plasma membrane and in part
through proteinaceous channels. Expression of the major intrinsic
protein (MIP) PIP1 in Xenopus laevis oocytes resulted in a 30% increase in the boron permeability of the oocytes. Other MIPs tested (PIP3, MLM1, and GlpF) did not have this effect. We
postulate that certain MIPs, like those that have recently been shown
to transport small neutral solutes, may also be the channels through
which boron enters plant cells.
1
This work was supported by the State
Scholarships Foundation (IKY) of Greece and by the U.S. Department of
Agriculture (grant no. 9801010).
2
Present address: Plant Science Department,
Faculty of Agriculture and Food Sciences, University of Manitoba,
Winnipeg, MB, Canada R3T 2N2.
*
Corresponding author; e-mail dordasc{at}cc.umanitoba.ca; fax
204-474-7528.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
B. Choat, G. A. Gambetta, K. A. Shackel, and M. A. Matthews
Vascular Function in Grape Berries across Development and Its Relevance to Apparent Hydraulic Isolation
Plant Physiology,
November 1, 2009;
151(3):
1677 - 1687.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G.-W. Li, M.-H. Zhang, W.-M. Cai, W.-N. Sun, and W.-A. Su
Characterization of OsPIP2;7, a Water Channel Protein in Rice
Plant Cell Physiol.,
December 1, 2008;
49(12):
1851 - 1858.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Tanaka, I. S. Wallace, J. Takano, D. M. Roberts, and T. Fujiwara
NIP6;1 Is a Boric Acid Channel for Preferential Transport of Boron to Growing Shoot Tissues in Arabidopsis
PLANT CELL,
October 1, 2008;
20(10):
2860 - 2875.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Mahdieh, A. Mostajeran, T. Horie, and M. Katsuhara
Drought Stress Alters Water Relations and Expression of PIP-Type Aquaporin Genes in Nicotiana tabacum Plants
Plant Cell Physiol.,
May 1, 2008;
49(5):
801 - 813.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Wei, E. Alexandersson, D. Golldack, A. J. Miller, P. O. Kjellbom, and W. Fricke
HvPIP1;6, a Barley (Hordeum vulgare L.) Plasma Membrane Water Channel Particularly Expressed in Growing Compared with Non-Growing Leaf Tissues
Plant Cell Physiol.,
August 1, 2007;
48(8):
1132 - 1147.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Jennings, T. R. Howren, J. Cui, M. Winters, and R. Hannigan
Transport and regulatory characteristics of the yeast bicarbonate transporter homolog Bor1p
Am J Physiol Cell Physiol,
July 1, 2007;
293(1):
C468 - C476.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Takano, M. Wada, U. Ludewig, G. Schaaf, N. von Wiren, and T. Fujiwara
The Arabidopsis Major Intrinsic Protein NIP5;1 Is Essential for Efficient Boron Uptake and Plant Development under Boron Limitation
PLANT CELL,
June 1, 2006;
18(6):
1498 - 1509.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Alleva, C. M. Niemietz, M. Sutka, C. Maurel, M. Parisi, S. D. Tyerman, and G. Amodeo
Plasma membrane of Beta vulgaris storage root shows high water channel activity regulated by cytoplasmic pH and a dual range of calcium concentrations
J. Exp. Bot.,
February 1, 2006;
57(3):
609 - 621.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. HUANG, Z. YE, R. W. BELL, and B. DELL
Boron Nutrition and Chilling Tolerance of Warm Climate Crop Species
Ann. Bot.,
October 1, 2005;
96(5):
755 - 767.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Hayes and R. J. Reid
Boron Tolerance in Barley Is Mediated by Efflux of Boron from the Roots
Plant Physiology,
October 1, 2004;
136(2):
3376 - 3382.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Moshelion, N. Moran, and F. Chaumont
Dynamic Changes in the Osmotic Water Permeability of Protoplast Plasma Membrane
Plant Physiology,
August 1, 2004;
135(4):
2301 - 2317.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Fetter, V. Van Wilder, M. Moshelion, and F. Chaumont
Interactions between Plasma Membrane Aquaporins Modulate Their Water Channel Activity
PLANT CELL,
January 1, 2004;
16(1):
215 - 228.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. JAVOT and C. MAUREL
The Role of Aquaporins in Root Water Uptake
Ann. Bot.,
September 1, 2002;
90(3):
301 - 313.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Moshelion, D. Becker, A. Biela, N. Uehlein, R. Hedrich, B. Otto, H. Levi, N. Moran, and R. Kaldenhoff
Plasma Membrane Aquaporins in the Motor Cells of Samanea saman: Diurnal and Circadian Regulation
PLANT CELL,
March 1, 2002;
14(3):
727 - 739.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
