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Plant Physiol, December 2000, Vol. 124, pp. 1582-1594
A New Set of Arabidopsis Expressed Sequence Tags from Developing
Seeds. The Metabolic Pathway from Carbohydrates to Seed
Oil1,[w]
Joseph A.
White,2
Jim
Todd,
Tom
Newman,
Nicole
Focks,
Thomas
Girke,
Oscar Martínez
de Ilárduya,
Jan
G.
Jaworski,
John B.
Ohlrogge, and
Christoph
Benning*
Departments of Biochemistry and Molecular Biology (J.A.W., N.F.,
C.B.), Botany and Plant Pathology (J.T., T.G., O.M.d.I., J.B.O.),
and United States Department of Energy-Plant Research Laboratory
(T.N.), Michigan State University, East Lansing, Michigan 48824; and
Department of Chemistry and Biochemistry, Miami University, Oxford,
Ohio 45056 (J.G.J.)
Large-scale single-pass sequencing of cDNAs from different plants
has provided an extensive reservoir for the cloning of genes, the
evaluation of tissue-specific gene expression, markers for map-based
cloning, and the annotation of genomic sequences. Although as of
January 2000 GenBank contained over 220,000 entries of expressed sequence tags (ESTs) from plants, most publicly available plant ESTs
are derived from vegetative tissues and relatively few ESTs are
specifically derived from developing seeds. However, important morphogenetic processes are exclusively associated with seed and embryo
development and the metabolism of seeds is tailored toward the
accumulation of economically valuable storage compounds such as oil.
Here we describe a new set of ESTs from Arabidopsis, which has been
derived from 5- to 13-d-old immature seeds. Close to 28,000 cDNAs have
been screened by DNA/DNA hybridization and approximately 10,500 new
Arabidopsis ESTs have been generated and analyzed using different
bioinformatics tools. Approximately 40% of the ESTs currently have no
match in dbEST, suggesting many represent mRNAs derived from genes that
are specifically expressed in seeds. Although these data can be mined
with many different biological questions in mind, this study emphasizes
the import of photosynthate into developing embryos, its conversion
into seed oil, and the regulation of this pathway.
1
This work was supported in parts by the National
Science Foundation (grant nos. MCB-94-06466 and IBN-97-23778), the
Midwestern Consortium for Plant Biotechnology Research, Dow
Agroscience, and the Michigan Agricultural Experiment Station.
2
Present address: The Institute for Genomic
Research, 9712 Medical Center Drive, Rockville, MD 20850.
[w]
The online version of this article contains Web-only
data. The supplemental material is available at www.plantphysiol.org.
*
Corresponding author; e-mail benning{at}pilot.msu.edu; fax
517-353-9334.
© 2000 American Society of Plant Physiologists
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