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Plant Physiol, December 2000, Vol. 124, pp. 1595-1604
Genetic Analysis of Seed-Soluble Oligosaccharides in Relation to
Seed Storability of Arabidopsis1
Leónie
Bentsink,
Carlos
Alonso-Blanco,2
Dick
Vreugdenhil,
Karine
Tesnier,
Steven P.C.
Groot, and
Maarten
Koornneef*
Laboratory of Genetics, Graduate School Experimental Plant Science,
Wageningen University, 2 Dreijenlaan 6703 HA Wageningen, The
Netherlands (L.B., C.A.-B., M.K.); Laboratory of Plant Physiology,
Graduate School Experimental Plant Science, Wageningen University, 6703 BD Wageningen, The Netherlands (D.V.); and Plant Research
International, Wageningen-University and Research Centre, NL-6700 AA
Wageningen, The Netherlands (K.T., S.P.C.G.)
Seed oligosaccharides (OSs) and especially raffinose series OSs
(RSOs) are hypothesized to play an important role in the acquisition of
desiccation tolerance and consequently in seed storability. In the
present work we analyzed the seed-soluble OS (sucrose, raffinose, and
stachyose) content of several Arabidopsis accessions and thus
identified the genotype Cape Verde Islands having a very low RSO
content. By performing quantitative trait loci (QTL) mapping in a
recombinant inbred line population, we found one major
QTL responsible for the practically monogenic segregation of seed stachyose content. This locus also affected the content of the two
other OSs, sucrose, and raffinose. Two candidate genes encoding respectively for galactinol synthase and raffinose synthase were located within the genomic region around this major QTL. In addition, three smaller-effect QTL were identified, each one specifically affecting the content of an individual OS. Seed storability was analyzed in the same recombinant inbred line population by measuring viability (germination) under two different seed aging assays: after
natural aging during 4 years of dry storage at room temperature and
after artificial aging induced by a controlled deterioration test.
Thus, four QTL responsible for the variation of this trait were mapped.
Comparison of the QTL genetic positions showed that the genomic region
containing the major OS locus did not significantly affect the seed
storability. We concluded that in the studied material neither RSOs nor
sucrose content had a specific effect on seed storability.
1
This work was supported by The Earth and Life
Sciences Foundation subsidized by The Netherlands Organization for
Scientific Research (to L.B.), by the Biotechnology Program of the
European Union (grant no. BIO4CT965008 to C.A.-B.), and by the
Agriculture and Fisheries Program of the European Community (grant no.
FAIR-BM-98-4743 to K.T.).
2
Present address: Centro Nacional de Biotecnologia,
Campus Universidad Autonoma, Cantoblanco, 28049 Madrid, Spain.
*
Corresponding author; e-mail
maarten.koornneef{at}genetics.dpw.wag-ur.nl; fax
31-317-483146.
© 2000 American Society of Plant Physiologists
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