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Plant Physiol, December 2000, Vol. 124, pp. 1648-1657
Pericycle Cell Proliferation and Lateral Root Initiation in
Arabidopsis1
Joseph G.
Dubrovsky,*
Peter W.
Doerner,2
Adán
Colón-Carmona,3 and
Thomas L.
Rost
Centro de Investigaciones Biológicas del Noroeste (CIBNOR),
Mar Bermejo, 195, Playa Palo Santa Rita, La Paz, Baja California Sur,
Mexico 23090 (J.G.D.); Section of Plant Biology, Division of Biological
Sciences, University of California, Davis, California 95616 (J.G.D.,
T.L.R.); and Plant Biology Laboratory, Salk Institute for Biological
Studies, P.O. Box 85800, San Diego, California 92186-5800 (P.W.D.,
A.C.C.)
In contrast with other cells generated by the root apical meristem
in Arabidopsis, pericycle cells adjacent to the
protoxylem poles of the vascular cylinder continue to cycle without
interruption during passage through the elongation and differentiation
zones. However, only some of the dividing pericycle cells are committed to the asymmetric, formative divisions that give rise to lateral root
primordia (LRPs). This was demonstrated by direct observation and mapping of mitotic figures, cell-length measurements, and the
histochemical analysis of a cyclin-GUS fusion protein in pericycle cells. The estimated duration of a pericycle cell cycle in the root
apical meristem was similar to the interval between cell displacement
from the meristem and the initiation of LRP formation. Developmentally
controlled LRP initiation occurs early, 3 to 8 mm from the root tip.
Thus the first growth control point in lateral root formation is
defined by the initiation of primordia in stochastic patterns by cells
passing through the elongation and young differentiation zones, up to
where lateral roots begin to emerge from the primary root. Therefore,
the first growth control point is not restricted to a narrow
developmental window. We propose that late LRP initiation is
developmentally unrelated to the root apical meristem and is operated
by a second growth control point that can be activated by environmental
cues. The observation that pericycle cells divide and lateral root
primordia form without intervening mitotic quiescence suggests that
lateral organ formation in roots and shoots might not be as
fundamentally different as previously thought.
1
This work was supported by a UCMEXUS
grant from the University of California. Work in the P.W.D. lab was
supported in part by the U.S. Department of Agriculture (grant no.
95-37304-2228). J.G.D. was supported by the Mexican Council for
Science and Technology during his sabbatical leave.
2
Present address: Institute for Cell and Molecular
Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK.
3
Present address: Department of Biology, University of
Massachusetts, Boston, MA 02125-3393.
*
Corresponding author; e-mail jdubrov{at}cibnor.mx; fax: 52-
112-54710.
© 2000 American Society of Plant Physiologists
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