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Plant Physiol, December 2000, Vol. 124, pp. 1658-1667
An Enhancer Trap Line Associated with a D-Class Cyclin Gene in
Arabidopsis1
Kankshita
Swaminathan,
Yingzhen
Yang,
Natasha
Grotz,
Lauren
Campisi, and
Thomas
Jack*
Department of Biological Sciences, Dartmouth College, Hanover, New
Hampshire 03755
In yeast and animals, cyclins have been demonstrated to be
important regulators of cell cycle progression. In recent years, a
large number of A-, B-, and D-class cyclins have been isolated from a
variety of plant species. One class of cyclins, the D-class cyclins, is
important for progression through G1 phase of the cell cycle. In
Arabidopsis, four D-class cyclins have been isolated and characterized
(CYCLIN-D1;1, CYCLIN-D2;1,
CYCLIN-D3;1, and CYCLIN-D4;1). In this
report we describe the characterization of a fifth D-class cyclin gene,
CYCLIN-D3;2 (CYCD3;2), from Arabidopsis. An enhancer trap line, line 5580, contains a T-DNA insertion in CYCD3;2. Enhancer trap line 5580 exhibits expression in
young vegetative and floral primordia. In line 5580, T-DNA is inserted in the first exon of the CYCD3;2 gene; in homozygous
5580 plants CYCD3;2 RNA is not detectable. Even though
CYCD3;2 gene function is eliminated, homozygous 5580 plants do not exhibit an obvious growth or developmental phenotype. Via
in situ hybridization we demonstrate that CYCD3;2 RNA is
expressed in developing vegetative and floral primordia. In addition,
CYCD3;2 is also capable of rescuing a yeast strain that
is deficient in G1 cyclin activity.
1
This work was supported by the U.S. Department
of Agriculture (grant no. 9602602).
*
Corresponding author; e-mail thomas.jack{at}dartmouth.edu; fax
603-646-1347.
© 2000 American Society of Plant Physiologists
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