|
Plant Physiol, December 2000, Vol. 124, pp. 1668-1677
Chloroplast Division and Morphology Are Differentially Affected
by Overexpression of FtsZ1 and FtsZ2 Genes
in Arabidopsis1,[w]
Kevin D.
Stokes,2
Rosemary S.
McAndrew,2
Rubi
Figueroa,
Stanislav
Vitha, and
Katherine W.
Osteryoung*
Department of Botany and Plant Pathology, 166 Plant Biology
Building, Michigan State University, East Lansing, Michigan 48824-1312
(K.D.S., R.S.M., S.V., K.W.O.); and Department of Biochemistry, Mail
Stop 200, University of Nevada, Reno, Nevada 89557 (R.F.)
In higher plants, two nuclear gene families, FtsZ1
and FtsZ2, encode homologs of the bacterial protein
FtsZ, a key component of the prokaryotic cell division machinery. We
previously demonstrated that members of both gene families are
essential for plastid division, but are functionally distinct. To
further explore differences between FtsZ1 and FtsZ2 proteins we
investigated the phenotypes of transgenic plants overexpressing
AtFtsZ1-1 or AtFtsZ2-1, Arabidopsis members of the FtsZ1 and FtsZ2 families,
respectively. Increasing the level of AtFtsZ1-1 protein as little as
3-fold inhibited chloroplast division. Plants with the most severe
plastid division defects had 13- to 26-fold increases in AtFtsZ1-1
levels over wild type, and some of these also exhibited a novel
chloroplast morphology. Quantitative immunoblotting revealed a
correlation between the degree of plastid division inhibition and the
extent to which the AtFtsZ1-1 protein level was elevated. In contrast,
expression of an AtFtsZ2-1 sense transgene had no
obvious effect on plastid division or morphology, though AtFtsZ2-1
protein levels were elevated only slightly over wild-type levels. This
may indicate that AtFtsZ2-1 accumulation is more tightly regulated than
that of AtFtsZ1-1. Plants expressing the AtFtsZ2-1
transgene did accumulate a form of the protein smaller than those
detected in wild-type plants. AtFtsZ2-1 levels were unaffected by
increased or decreased accumulation of AtFtsZ1-1 and vice versa,
suggesting that the levels of these two plastid division proteins are
regulated independently. Taken together, our results provide additional
evidence for the functional divergence of the FtsZ1 and
FtsZ2 plant gene families.
1
This work was supported by the U.S. National
Science Foundation (grant no. MCB-9604412) and by the Nevada
Agricultural Experiment Station.
2
These authors contributed equally to this work.
[w]
Indicates Web-only data.
*
Corresponding author; osteryou{at}msu.edu; fax 517-353-1926.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
L. Y. Tang, N. Nagata, R. Matsushima, Y. Chen, Y. Yoshioka, and W. Sakamoto
Visualization of Plastids in Pollen Grains: Involvement of FtsZ1 in Pollen Plastid Division
Plant Cell Physiol.,
April 1, 2009;
50(4):
904 - 908.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Vitova, J. Hendrychova, M. Cizkova, V. Cepak, J. G. Umen, V. Zachleder, and K. Bisova
Accumulation, Activity and Localization of Cell Cycle Regulatory Proteins and the Chloroplast Division Protein FtsZ in the Alga Scenedesmus quadricauda under Inhibition of Nuclear DNA Replication
Plant Cell Physiol.,
December 1, 2008;
49(12):
1805 - 1817.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. W. Yoder, D. Kadirjan-Kalbach, B. J. S. C. Olson, S.-y. Miyagishima, S. L. DeBlasio, R. P. Hangarter, and K. W. Osteryoung
Effects of Mutations in Arabidopsis FtsZ1 on Plastid Division, FtsZ Ring Formation and Positioning, and FtsZ Filament Morphology in Vivo
Plant Cell Physiol.,
June 1, 2007;
48(6):
775 - 791.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Maple and S. G. Moller
Plastid Division: Evolution, Mechanism and Complexity
Ann. Bot.,
April 1, 2007;
99(4):
565 - 579.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Gao, T. L. Sage, and K. W. Osteryoung
FZL, an FZO-like protein in plants, is a determinant of thylakoid and chloroplast morphology
PNAS,
April 25, 2006;
103(17):
6759 - 6764.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Aldridge, J. Maple, and S. G. Moller
The molecular biology of plastid division in higher plants
J. Exp. Bot.,
April 1, 2005;
56(414):
1061 - 1077.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Constan, J. E. Froehlich, S. Rangarajan, and K. Keegstra
A Stromal Hsp100 Protein Is Required for Normal Chloroplast Development and Function in Arabidopsis
Plant Physiology,
November 1, 2004;
136(3):
3605 - 3615.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Raynaud, C. Cassier-Chauvat, C. Perennes, and C. Bergounioux
An Arabidopsis Homolog of the Bacterial Cell Division Inhibitor SulA Is Involved in Plastid Division
PLANT CELL,
July 1, 2004;
16(7):
1801 - 1811.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. W. Osteryoung and J. Nunnari
The Division of Endosymbiotic Organelles
Science,
December 5, 2003;
302(5651):
1698 - 1704.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Vitha, J. E. Froehlich, O. Koksharova, K. A. Pyke, H. van Erp, and K. W. Osteryoung
ARC6 Is a J-Domain Plastid Division Protein and an Evolutionary Descendant of the Cyanobacterial Cell Division Protein Ftn2
PLANT CELL,
August 1, 2003;
15(8):
1918 - 1933.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Wang, D. Kong, Y. Wang, Y. Hu, Y. He, and J. Sun
Isolation of two plastid division ftsZ genes from Chlamydomonas reinhardtii and its evolutionary implication for the role of FtsZ in plastid division
J. Exp. Bot.,
March 1, 2003;
54(384):
1115 - 1116.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. J. Jeong, Y.-I. Park, K. Suh, J. A. Raven, O. J. Yoo, and J. R. Liu
A Large Population of Small Chloroplasts in Tobacco Leaf Cells Allows More Effective Chloroplast Movement Than a Few Enlarged Chloroplasts
Plant Physiology,
May 1, 2002;
129(1):
112 - 121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Itoh, M. Fujiwara, N. Nagata, and S. Yoshida
A Chloroplast Protein Homologous to the Eubacterial Topological Specificity Factor MinE Plays a Role in Chloroplast Division
Plant Physiology,
December 1, 2001;
127(4):
1644 - 1655.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. S. McAndrew, J. E. Froehlich, S. Vitha, K. D. Stokes, and K. W. Osteryoung
Colocalization of Plastid Division Proteins in the Chloroplast Stromal Compartment Establishes a New Functional Relationship between FtsZ1 and FtsZ2 in Higher Plants
Plant Physiology,
December 1, 2001;
127(4):
1656 - 1666.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Mori, H. Kuroiwa, M. Takahara, S.-y. Miyagishima, and T. Kuroiwa
Visualization of an FtsZ Ring in Chloroplasts of Lilium longiflorum Leaves
Plant Cell Physiol.,
June 1, 2001;
42(6):
555 - 559.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Vitha, R. S. McAndrew, and K. W. Osteryoung
FtsZ Ring Formation at the Chloroplast Division Site in Plants
J. Cell Biol.,
April 2, 2001;
153(1):
111 - 120.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
