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Plant Physiol, December 2000, Vol. 124, pp. 1766-1774
Pathogenesis of the Human Opportunistic Pathogen
Pseudomonas aeruginosa PA14 in
Arabidopsis1
Julia M.
Plotnikova,
Laurence G.
Rahme, and
Frederick M.
Ausubel*
Departments of Genetics (J.M.P., F.M.A.) and Surgery (L.G.R.),
Harvard Medical School, Boston, Massachusetts 02115; and Department of
Molecular Biology (J.M.P., F.M.A.), Massachusetts General Hospital,
Boston, Massachusetts 02114
The human opportunistic pathogen Pseudomonas
aeruginosa strain PA14 is a multihost pathogen that can infect
Arabidopsis. We found that PA14 pathogenesis in Arabidopsis involves
the following steps: attachment to the leaf surface, congregation of
bacteria at and invasion through stomata or wounds, colonization of
intercellular spaces, and concomitant disruption of plant cell wall and
membrane structures, basipetal movement along the vascular parenchyma, and maceration and rotting of the petiole and central bud. Distinctive features of P. aeruginosa pathogenesis are that the
surface of mesophyll cell walls adopt an unusual convoluted or
undulated appearance, that PA14 cells orient themselves perpendicularly to the outer surface of mesophyll cell walls, and that PA14 cells make
circular perforations, approximately equal to the diameter of P.
aeruginosa, in mesophyll cell walls. Taken together, our data
show that P. aeruginosa strain PA14 is a facultative
pathogen of Arabidopsis that is capable of causing local and systemic
infection, which can result in the death of the infected plant.
1
This work was supported by the National
Institutes of Health (grant no. GM48707 to F.M.A.) and by a grant from
Aventis SA to Massachusetts General Hospital.
*
Corresponding author; ausubel{at}frodo.mgh.harvard.edu; fax
617-726-5949.
© 2000 American Society of Plant Physiologists
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