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Plant Physiol, December 2000, Vol. 124, pp. 1844-1853
Interaction Specificity of Arabidopsis Calcineurin B-Like Calcium
Sensors and Their Target Kinases
Kyung-Nam
Kim,
Yong Hwa
Cheong,
Rajeev
Gupta, and
Sheng
Luan*
Department of Plant and Microbial Biology, University of
California, Berkeley, California 94720
Calcium is a critical component in a number of plant signal
transduction pathways. A new family of calcium sensors called calcineurin B-like proteins (AtCBLs) have been recently identified from
Arabidopsis. These calcium sensors have been shown to interact with a
family of protein kinases (CIPKs). Here we report that each individual
member of AtCBL family specifically interacts with a subset of CIPKs
and present structural basis for the interaction and for the
specificity underlying these interactions. Although the C-terminal
region of CIPKs is responsible for interaction with AtCBLs, the
N-terminal region of CIPKs is also involved in determining the
specificity of such interaction. We have also shown that all three
EF-hand motifs in AtCBL members are required for the interaction
with CIPKs. Several AtCBL members failed to interact with any of the
CIPKs presented in this study, suggesting that these AtCBL members
either have other CIPKs as targets or they target distinct proteins
other than CIPKs. These results may provide structural basis for the
functional specificity of CBL family of calcium sensors and their targets.
*
Corresponding author; e-mail sluan{at}nature.berkeley.edu; fax
510-642-4995.
© 2000 American Society of Plant Physiologists
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