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Plant Physiol, January 2001, Vol. 125, pp. 278-291

Oligoguluronates Elicit an Oxidative Burst in the Brown Algal Kelp Laminaria digitata1

Frithjof Christian Küpper, Bernard Kloareg, Jean Guern, and Philippe Potin*

Unité Mixte de Recherche, 1931 du Centre National de la Recherche Scientifique, and Laboratoires Goëmar, Station Biologique, Place G. Teissier, BP 74, F-29682 Roscoff, Brittany, France (F.C.K., B.K., P.P.); and Institut des Sciences Végétales, Centre National de la Recherche Scientifique, avenue de la Terrasse, F-91198 Gif-sur-Yvette, France (J.G.)

Oligomeric degradation products of alginate elicited a respiratory and oxidative burst in the sporophytes of the kelp Laminaria digitata. The generation of activated oxygen species (AOS), O2-, and H2O2 was detected at the single cell level, using nitroblue tetrazolium precipitation and a redox-sensitive fluorescent probe, respectively. The oxidative burst involved diphenyleneiodonium-sensitive AOS-generating machinery and its amplitude depended on the type of tissue. After a first elicitation plants were desensitized for about 3 h. The activity of alginate oligosaccharides was dose dependent, saturating around 40 µM. It was also structure-dependent, with homopolymeric blocks of alpha -1,4-L-guluronic acid, i.e. the functional analogs of oligogalacturonic blocks in pectins, being the most active signals. The perception of oligoguluronate signals resulted in a strong efflux of potassium. Pharmacological dissection of the early events preceding the emission of AOS indicated that the transduction chain of oligoguluronate signals in L. digitata is likely to feature protein kinases, phospholipase A2, as well as K+, Ca2+, and anion channels.

1 This work was supported by the Fonds Européens de Développement Régional (agricultural region; Vb) program of the European Commission. F.C.K. was generously supported with fellowships by Studienstiftung des Deutschen Volkes (Bonn), Hüls AG-Stiftung (Marl, Germany), and the European Commission (Program MAST-III, Brussels).

* Corresponding author; e-mail potin{at}sb-roscoff.fr; fax 33-2-98-29-23-24.

© 2001 American Society of Plant Physiologists


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