Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (22)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marcus, A. I.
Right arrow Articles by Cyr, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marcus, A. I.
Right arrow Articles by Cyr, R. J.
Agricola
Right arrow Articles by Marcus, A. I.
Right arrow Articles by Cyr, R. J.

Plant Physiol, January 2001, Vol. 125, pp. 387-395

The Role of Microtubules in Guard Cell Function1

Adam I. Marcus, Richard C. Moore,2 and Richard J. Cyr*

Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802

Guard cells are able to sense a multitude of environmental signals and appropriately adjust the stomatal pore to regulate gas exchange in and out of the leaf. The role of the microtubule cytoskeleton during these stomatal movements has been debated. To help resolve this debate, in vivo stomatal aperture assays with different microtubule inhibitors were performed. We observed that guard cells expressing the microtubule-binding green fluorescent fusion protein (green fluorescent protein::microtubule binding domain) fail to open for all major environmental triggers of stomatal opening. Furthermore, guard cells treated with the anti-microtubule drugs, propyzamide, oryzalin, and trifluralin also failed to open under the same environmental conditions. The inhibitory conditions caused by green fluorescent protein::microtubule binding domain and these anti-microtubule drugs could be reversed using the proton pump activator, fusicoccin. Therefore, we conclude that microtubules are involved in an upstream event prior to the ionic fluxes leading to stomatal opening. In a mechanistic manner, evidence is presented to implicate a microtubule-associated protein in this putative microtubule-based signal transduction event.

1 This work was supported by the U.S. Department of Agriculture (grant no. 98-35304-6658) and by the U.S. Department of Energy (grant no. DE-FG02-91ER20050).

2 Present address: University of North Carolina, Biology Department, 107 Coker Hall, Chapel Hill, NC 27599.

* Corresponding author; e-mail rjc8{at}psu.edu; fax 814-865-9131.

© 2001 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
P. Ruggenthaler, D. Fichtenbauer, J. Krasensky, C. Jonak, and E. Waigmann
Microtubule-Associated Protein AtMPB2C Plays a Role in Organization of Cortical Microtubules, Stomata Patterning, and Tobamovirus Infectivity
Plant Physiology, March 1, 2009; 149(3): 1354 - 1365.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. Lu, Z. Gong, J. Wang, J. Zhang, and J. Liang
Microtubule dynamics in relation to osmotic stress-induced ABA accumulation in Zea mays roots
J. Exp. Bot., July 1, 2007; 58(10): 2565 - 2572.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. C. Shope and K. A. Mott
Membrane trafficking and osmotically induced volume changes in guard cells
J. Exp. Bot., December 1, 2006; 57(15): 4123 - 4131.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
N. Kanzawa, Y. Hoshino, M. Chiba, D. Hoshino, H. Kobayashi, N. Kamasawa, Y. Kishi, M. Osumi, M. Sameshima, and T. Tsuchiya
Change in the Actin Cytoskeleton during Seismonastic Movement of Mimosa pudica
Plant Cell Physiol., April 1, 2006; 47(4): 531 - 539.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. I. Prokhnevsky, V. V. Peremyslov, and V. V. Dolja
Actin Cytoskeleton Is Involved in Targeting of a Viral Hsp70 Homolog to the Cell Periphery
J. Virol., November 15, 2005; 79(22): 14421 - 14428.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Emi, T. Kinoshita, K. Sakamoto, Y. Mineyuki, and K.-i. Shimazaki
Isolation of a Protein Interacting with Vfphot1a in Guard Cells of Vicia faba
Plant Physiology, July 1, 2005; 138(3): 1615 - 1626.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Lahav, M. Abu-Abied, E. Belausov, A. Schwartz, and E. Sadot
Microtubules of Guard Cells are Light Sensitive
Plant Cell Physiol., May 15, 2004; 45(5): 573 - 582.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Dhonukshe and T. W. J. Gadella Jr.
Alteration of Microtubule Dynamic Instability during Preprophase Band Formation Revealed by Yellow Fluorescent Protein-CLIP170 Microtubule Plus-End Labeling
PLANT CELL, March 1, 2003; 15(3): 597 - 611.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G. Komis, P. Apostolakos, and B. Galatis
Hyperosmotic Stress Induces Formation of Tubulin Macrotubules in Root-Tip Cells of Triticum turgidum: Their Probable Involvement in Protoplast Volume Control
Plant Cell Physiol., August 15, 2002; 43(8): 911 - 922.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. C. Gardiner, J. D. I. Harper, N. D. Weerakoon, D. A. Collings, S. Ritchie, S. Gilroy, R. J. Cyr, and J. Marc
A 90-kD Phospholipase D from Tobacco Binds to Microtubules and the Plasma Membrane
PLANT CELL, September 1, 2001; 13(9): 2143 - 2158.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2001 by the American Society of Plant Biologists